Anti-IRS1 (phospho S1101)抗体(ab55343)

概述

  • 产品名称Anti-IRS1 (phospho S1101)抗体
    参阅全部 IRS1 一抗
  • 描述
    兔多克隆抗体to IRS1 (phospho S1101)
  • 特异性This antibody detects endogenous levels of IRS1 only when phosphorylated at serine 1101.
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from human IRS1 around the phosphorylation site of serine 1101 (H-S-SP-E-T).

  • 阳性对照
    • Human colon carcinoma tissue, extracts from COS7 cells.

性能

应用

Our Abpromise guarantee covers the use of ab55343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
IHC-P
  • 应用说明ELISA: 1/5000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Detects a band of approximately 133 kDa (predicted molecular weight for Green monkey: 133 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
    • 疾病相关Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
    • 序列相似性Contains 1 IRS-type PTB domain.
      Contains 1 PH domain.
    • 翻译后修饰Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
      Phosphorylation of Tyr-896 is required for GRB2-binding.
    • Information by UniProt
    • 数据库链接
    • 别名
      • HIRS 1 antibody
      • HIRS1 antibody
      • Insulin receptor substrate 1 antibody
      • IRS 1 antibody
      • IRS-1 antibody
      • IRS1 antibody
      • IRS1_HUMAN antibody
      • OTTHUMP00000164234 antibody
      see all

    Anti-IRS1 (phospho S1101) antibody 图像

    • This image shows human colon carcinoma tissue stained with ab55343 at 1/50 dilution. The left hand image shows untreated tissue; the right hand image shows tissue treated with the immunising phosphopeptide.
    • All lanes : Anti-IRS1 (phospho S1101) antibody (ab55343) at 1/500 dilution

      Lane 1 : Extracts from COS7 cells treated with Calyculin A at 50ng/ml for 30 mins.
      Lane 2 : Extracts from COS7 cells treated with Calyculin A at 50ng/ml for 30 mins and with the immunising phosphopeptide.


      Predicted band size : 133 kDa
      Observed band size : 133 kDa

    Anti-IRS1 (phospho S1101) antibody (ab55343)参考文献

    ab55343 has not yet been referenced specifically in any publications.

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