重组Anti-IRS1+IRS2抗体[EP263Y] (ab40777)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP263Y] to IRS1 + IRS2
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-IRS1+IRS2抗体[EP263Y]
参阅全部 IRS1+IRS2 一抗 -
描述
兔单克隆抗体[EP263Y] to IRS1 + IRS2 -
宿主
Rabbit -
特异性
The immunogen used for this product shares 78% homology with IRS2. Cross-reactivity with this protein has not been confirmed experimentally.
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经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IF, IHC-Pmore details
不适用于: IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human IRS1 (C terminal). The exact sequence is proprietary.
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阳性对照
- WB: HeLa and HEK293 cell lysates, HEK-293T transfected with a human IRS1 expression vector containing a Myc/DDK tag whole cell lysate, HEK-293T transfected with a human IRS2 expression vector containing a Myc/DDK tag whole cell lysate. IHC-P: Human breast carcinoma and cervical carcinoma tissues. ICC/IF: MCF-7 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP263Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab40777于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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WB |
1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 130 kDa).
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ICC/IF |
1/250 - 1/500.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
WB
1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 130 kDa). |
ICC/IF
1/250 - 1/500. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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细胞定位
IRS2: Cytoplasm > cytosol. -
数据库链接
- Entrez Gene: 3667 Human
- Entrez Gene: 8660 Human
- Omim: 147545 Human
- Omim: 600797 Human
- SwissProt: P35568 Human
- SwissProt: Q9Y4H2 Human
- Unigene: 442344 Human
- Unigene: 471508 Human
图片
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Lanes 1-3 : Anti-IRS1+IRS2 antibody [EP263Y] (ab40777) at 1/1000 dilution
Lanes 4-6 : Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab205606) at 1/1000 dilution
Lanes 1 & 4 : HEK-293T transfected with an empty vector whole cell lysate
Lanes 2 & 5 : HEK-293T transfected with a human IRS1 expression vector containing a Myc/DDK tag whole cell lysate
Lanes 3 & 6 : HEK-293T transfected with a human IRS2 expression vector containing a Myc/DDK tag whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 130 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM /TBST.
Exposure time: Lane 1, 2, 4, 5 , 6: 1 second. Lane 3: 80 seconds.
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Anti-IRS1+IRS2 antibody [EP263Y] (ab40777) at 1/10000 dilution (purified) + HEK293 whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 130 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Blocking and dilution buffer: 5% NFDM/TBST -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labelling IRS1 with purified ab40777 at a dilution of 1/100. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labelling IRS1 with purified ab40777 at a dilution of 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/250) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
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Anti-IRS1+IRS2 antibody [EP263Y] (ab40777) (purified) + HeLa whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 130 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Blocking and dilution buffer: 5% NFDM/TBST -
Intracellular Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling IRS1 with unpurified ab40777 at 1/90 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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Anti-IRS1+IRS2 antibody [EP263Y] (ab40777) at 1/1000 dilution (unpurified) + HeLa cell lysate at 10 µg
Predicted band size: 130 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma labelling IRS1 with unpurified ab40777.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (20)
ab40777 被引用在 20 文献中.
- Liu X et al. β-Carotene regulates glucose transport and insulin resistance in gestational diabetes mellitus by increasing the expression of SHBG. Clin Exp Pharmacol Physiol 49:1307-1318 (2022). PubMed: 35986631
- Pan S et al. STIM1 promotes angiogenesis by reducing exosomal miR-145 in breast cancer MDA-MB-231 cells. Cell Death Dis 12:38 (2021). PubMed: 33414420
- Zhao L et al. TNF-a promotes insulin resistance in obstructive sleep apnea-hypopnea syndrome. Exp Ther Med 21:568 (2021). PubMed: 33850540
- Su C et al. Roux-en-Y Gastric Bypass Improves Hepatic Glucose Metabolism Involving Upregulation of Sirt1 in Type 2 Diabetes Mellitus. Diabetes Metab Syndr Obes 14:2269-2280 (2021). PubMed: 34045876
- Eyler CE et al. Single-cell lineage analysis reveals genetic and epigenetic interplay in glioblastoma drug resistance. Genome Biol 21:174 (2020). PubMed: 32669109