概述

  • 产品名称Anti-IRS1抗体
    参阅全部 IRS1 一抗
  • 描述
    兔多克隆抗体to IRS1
  • 特异性ab52167 detects endogenous levels of total IRS1 protein.
  • 经测试应用适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic non-phosphopeptide derived from human IRS1 around the phosphorylation site of serine 307 (T-E-S-I-T).

  • 阳性对照
    • Human breast carcinoma tissue and A431 cell extarcts.

性能

应用

Our Abpromise guarantee covers the use of ab52167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 132 kDa.
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 5 µg/ml.

靶标

  • 功能May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
  • 疾病相关Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
  • 序列相似性Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • 翻译后修饰Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
    Phosphorylation of Tyr-896 is required for GRB2-binding.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HIRS 1 antibody
    • HIRS1 antibody
    • Insulin receptor substrate 1 antibody
    • IRS 1 antibody
    • IRS-1 antibody
    • IRS1 antibody
    • IRS1_HUMAN antibody
    • OTTHUMP00000164234 antibody
    see all

Anti-IRS1 antibody 图像

  • All lanes : Anti-IRS1 antibody (ab52167) at 1/500 dilution

    Lane 1 : A431 cell extract
    Lane 2 : A431 cell extract with immunising peptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 132 kDa
    Observed band size : >175 kDa (why is the actual band size different from the predicted?)
  • ab52167 at 1/50 dilution staining IRS1 in human breast carcinoma by Immunohistochemsitry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
  • ICC/IF image of ab52167 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52167, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-IRS1 antibody (ab52167)参考文献

This product has been referenced in:

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"