Anti-Iroquois homeobox protein 3抗体- ChIP Grade (ab25703)

概述

  • 产品名称Anti-Iroquois homeobox protein 3抗体- ChIP Grade
    参阅全部 Iroquois homeobox protein 3 一抗
  • 描述
    兔多克隆抗体to Iroquois homeobox protein 3 - ChIP Grade
  • 经测试应用适用于: WB, IHC-Fr, ChIPmore details
  • 种属反应性
    与反应: Mouse, Chicken
    预测可用于: Human, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Mouse Iroquois homeobox protein 3.

    (Peptide available as ab26906.)

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lystaes: Mouse normal lung; Mouse normal brain; Mouse normal spinal cord and Mouse 14-day fetus.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab25703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Detects a band of approximately 52, 55 kDa (predicted molecular weight: 52 kDa).
IHC-Fr 1/100.
ChIP Use at an assay dependent concentration. PubMed: 22992950

靶标

Anti-Iroquois homeobox protein 3 antibody - ChIP Grade 图像

  • All lanes : Anti-Iroquois homeobox protein 3 antibody - ChIP Grade (ab25703) at 2 µg/ml

    Lane 1 : Mouse Fetus (14 Day Old) Tissue Lysate
    Lane 2 : Mouse lung normal tissue lysate - total protein (ab29297)
    Lane 3 : Brain (Mouse) Tissue Lysate
    Lane 4 : Spinal Cord (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 52,55 kDa (why is the actual band size different from the predicted?)

Anti-Iroquois homeobox protein 3 antibody - ChIP Grade (ab25703)参考文献

This product has been referenced in:
  • Gaborit N  et al. Cooperative and antagonistic roles for Irx3 and Irx5 in cardiac morphogenesis and postnatal physiology. Development 139:4007-19 (2012). ChIP ; Mouse . Read more (PubMed: 22992950) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Embryonic ovary, lung, limb)
Specification Embryonic ovary, lung, limb
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Triton-X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Aug 02 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Chicken Tissue sections (Neural tube)
Specification Neural tube
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 10%
Username

Dr. Katrin Götz

Verified customer

提交于 Apr 23 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"