The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Transcription factor. Involved in SHH-dependent neural patterning. Together with NKX2-2 and NKX6-1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class I proteins of neuronal progenitor factors, which are repressed by SHH signals. Involved in the transcriptional repression of MNX1 in non-motor neuron cells.
Belongs to the TALE/IRO homeobox family. Contains 1 homeobox DNA-binding domain.
ab111180 at 1/50 dilution staining Iroquois homeobox protein 3 in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry. The image on the right is treated with the synthesized peptide