概述
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产品名称Anti-IRGM抗体
参阅全部 IRGM 一抗 -
描述兔多克隆抗体to IRGM
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经测试应用适用于: WB, ELISA, IHC-Pmore details
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种属反应性与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide corresponding to 14 amino acids from near the centre of Human IRGM.
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阳性对照
- Human Colon and Brain tissue.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
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存储溶液Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab118569 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 20 kDa. | |
| ELISA | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能Putative GTPase which is required for clearance of acute protozoan and bacterial infections. Functions in innate immune response probably through regulation of autophagy. May regulate proinflammatory cytokine production and prevent endotoxemia upon infection. May also play a role in macrophages adhesion and motility.
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组织特异性Widely expressed (at protein level). Expressed in several tissues including colon, small bowel and peripheral blood leukocytes.
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疾病相关Defects in IRGM are the cause of susceptibility to inflammatory bowel disease type 19 (IBD19) [MIM:612278]. A chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
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序列相似性Belongs to the interferon-inducible GTPase family.
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细胞定位Golgi apparatus membrane. Cell membrane. Cytoplasmic vesicle > phagosome membrane. Cytoplasmic vesicle > autophagosome membrane. Cell projection > phagocytic cup. Behaves like an integral membrane protein (By similarity). Recruited to the plasma membrane around forming phagocytic cups, it remains associated with maturing autophagosomes (By similarity). Preferentially associated with cis- and medial-Golgi.
- Information by UniProt
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数据库链接
- Entrez Gene: 345611 Human
- Entrez Gene: 15944 Mouse
- Entrez Gene: 303090 Rat
- SwissProt: A1A4Y4 Human
- SwissProt: Q60766 Mouse
- SwissProt: Q6AYC2 Rat
- Unigene: 519680 Human
- Unigene: 652148 Human
see all -
别名
- IFI1 antibody
- Iigp3 antibody
- Iipg3 antibody
see all
Anti-IRGM antibody 图像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRGM antibody (ab118569)Human Colon (formalin-fixed, paraffin-embedded) stained with IRGM antibody ab118569 at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. -
Western blot - Anti-IRGM antibody (ab118569)Lane 1 : Anti-IRGM antibody (ab118569) at 1 µg/ml
Lane 2 : Anti-IRGM antibody (ab118569) at 2 µg/ml
Lane 1 : Human Brain lysate
Lane 2 : Human Brain lysate
Predicted band size : 20 kDa
Anti-IRGM antibody (ab118569)参考文献
ab118569 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"