Anti-IRGM抗体[403CT8.5.5] (ab63230)

概述

  • 产品名称Anti-IRGM抗体[403CT8.5.5]
    参阅全部 IRGM 一抗
  • 描述
    小鼠单克隆抗体[403CT8.5.5] to IRGM
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Full-length recombinant mouse IRGM protein.

  • 阳性对照
    • This antibody gave a positive signal in mouse brain and mouse heart tissue lysates.

性能

应用

Our Abpromise guarantee covers the use of ab63230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). Block using 3% milk.

靶标

  • 功能Putative GTPase which is required for clearance of acute protozoan and bacterial infections. Functions in innate immune response probably through regulation of autophagy. May regulate proinflammatory cytokine production and prevent endotoxemia upon infection. May also play a role in macrophages adhesion and motility.
  • 组织特异性Widely expressed (at protein level). Expressed in several tissues including colon, small bowel and peripheral blood leukocytes.
  • 疾病相关Defects in IRGM are the cause of susceptibility to inflammatory bowel disease type 19 (IBD19) [MIM:612278]. A chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
  • 序列相似性Belongs to the interferon-inducible GTPase family.
  • 细胞定位Golgi apparatus membrane. Cell membrane. Cytoplasmic vesicle > phagosome membrane. Cytoplasmic vesicle > autophagosome membrane. Cell projection > phagocytic cup. Behaves like an integral membrane protein (By similarity). Recruited to the plasma membrane around forming phagocytic cups, it remains associated with maturing autophagosomes (By similarity). Preferentially associated with cis- and medial-Golgi.
  • Information by UniProt
  • 数据库链接
  • 别名
    • IFI1 antibody
    • Iigp3 antibody
    • Iipg3 antibody
    • Immunity related GTPase family M protein 1 antibody
    • Immunity related GTPase family, M antibody
    • Immunity-related GTPase family M protein 1 antibody
    • Immunity-related GTPase family M protein antibody
    • Interferon inducible protein 1 antibody
    • Interferon-inducible protein 1 antibody
    • Irgm antibody
    • IRGM_HUMAN antibody
    • IRGM1 antibody
    • LPS-stimulated RAW 264.7 macrophage protein 47 homolog antibody
    • LRG 47 antibody
    • LRG-47 antibody
    • LRG-47-like protein antibody
    • LRG47 antibody
    • MGC149263 antibody
    • MGC149264 antibody
    see all

Anti-IRGM antibody [403CT8.5.5] 图像

  • All lanes : Anti-IRGM antibody [403CT8.5.5] (ab63230) at 1/100 dilution

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Heart (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Peroxidase- conjugated AffiniPure Goat Anti-mouse IgM at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 45,47 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

Anti-IRGM antibody [403CT8.5.5] (ab63230)参考文献

ab63230 has not yet been referenced specifically in any publications.

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