概述

  • 产品名称Anti-IRF8抗体[mAbcam61750]
    参阅全部 IRF8 一抗
  • 描述
    小鼠单克隆抗体[mAbcam61750] to IRF8
  • 经测试应用适用于: WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
    预测可用于: Orangutan
  • 免疫原

    Recombinant fragment derived from within residues 100 - 200 of Human IRF8.

  • 阳性对照
    • This antibody gave a positive signal in Jurkat and K562 whole cell and nuclear lysates.
  • 常规说明

    We can conjugate this antibody to FITC for you (please see ab150224 for details).   This antibody is available as an azide-free product (see ab174642).

性能

应用

Our Abpromise guarantee covers the use of ab61750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 10 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system.
  • 组织特异性Predominantly in lymphoid tissues.
  • 序列相似性Belongs to the IRF family.
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
  • 翻译后修饰Ubiquitinated. Ubiquitination by TRIM21 in macrophages, a process that is strongly increased upon interferon gamma stimulation, leds to the enhanced transcriptional activity of target cytokine genes.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • H ICSBP antibody
    • H-ICSBP antibody
    • HGNC:5358 antibody
    • HICSBP antibody
    • ICSBP 1 antibody
    • ICSBP antibody
    • ICSBP1 antibody
    • Interferon consensus sequence binding protein 1 antibody
    • Interferon consensus sequence binding protein antibody
    • Interferon consensus sequence-binding protein antibody
    • Interferon regulatory factor 8 antibody
    • IRF 8 antibody
    • IRF-8 antibody
    • Irf8 antibody
    • IRF8_HUMAN antibody
    • MYLS antibody
    see all

Anti-IRF8 antibody [mAbcam61750] 图像

  • All lanes : Anti-IRF8 antibody [mAbcam61750] (ab61750) at 10 µg/ml

    Lane 1 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Nuclear Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 4 : Jurkat nuclear extract lysate (ab14844)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 13 kDa,17 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes
  • Overlay histogram showing K562 cells stained with ab61750 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab61750, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-IRF8 antibody [mAbcam61750] (ab61750)参考文献

This product has been referenced in:
  • Suzuki M  et al. Aberrant methylation and silencing of IRF8 expression in non-small cell lung cancer. Oncol Lett 8:1025-1030 (2014). IHC-P ; Human . Read more (PubMed: 25120651) »

See 1 Publication for this product

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