Anti-IRF8抗体(ab3428)

概述

  • 产品名称
  • 描述
    山羊多克隆抗体to IRF8
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide: QRSFFRENQQITV, corresponding to C terminal amino acids 414-426 of Human ICSBP.

  • 阳性对照
    • K562 cell lysate
  • 常规说明
    Principal Names – ICSBP1; IRF8; ICSBP; IRF-8; interferon consensus sequence binding protein 1; H-ICSBP; interferon regulatory factor 8. Official Gene Symbol - ICSBP1. GenBank Accession Number – NP_002154. LocusLink ID - 3394 (human); 15900 (mouse). Gene Ontology terms - RNA polymerase II transcription factor activity; RNA polymerase II transcription factor activity, enhancer binding; immune response; negative regulation of transcription from Pol II promoter.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    pH: 7.30
    Constituents: 0.5% Tris, 0.5% BSA
  • Concentration information loading...
  • 纯化说明
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明
    WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 47 kDa in K562 lysate(predicted molecular weight: 47 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system.
    • 组织特异性
      Predominantly in lymphoid tissues.
    • 序列相似性
      Belongs to the IRF family.
      Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
    • 翻译后修饰
      Ubiquitinated. Ubiquitination by TRIM21 in macrophages, a process that is strongly increased upon interferon gamma stimulation, leds to the enhanced transcriptional activity of target cytokine genes.
    • 细胞定位
      Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • H ICSBP antibody
      • H-ICSBP antibody
      • HGNC:5358 antibody
      • HICSBP antibody
      • ICSBP 1 antibody
      • ICSBP antibody
      • ICSBP1 antibody
      • Interferon consensus sequence binding protein 1 antibody
      • Interferon consensus sequence binding protein antibody
      • Interferon consensus sequence-binding protein antibody
      • Interferon regulatory factor 8 antibody
      • IRF 8 antibody
      • IRF-8 antibody
      • Irf8 antibody
      • IRF8_HUMAN antibody
      • MYLS antibody
      see all

    Anti-IRF8 antibody 图像



    • Predicted band size : 47 kDa


      ab3428 at a 1µg/ml concentration staining ICSBP in K562 lysate (RIPA buffer, 30 µg total protein per lane, 1 hour incubation). Detected by Western blot (ECL).

    实验方案

    Anti-IRF8 antibody (ab3428)参考文献

    ab3428 has not yet been referenced specifically in any publications.

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