重组
RabMAb

Anti-IRF7抗体[EPR4718] (ab109255)

概述

  • 产品名称
    Anti-IRF7抗体[EPR4718]
    参阅全部 IRF7 一抗
  • 描述
    兔单克隆抗体[EPR4718] to IRF7
  • 经测试应用
    适用于: WB, IP, Flow Cyt, IHC-P, IHC-Fr, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human IRF7 aa 200-300.

  • 阳性对照
    • Jurkat, Raji, HuT78 and RAW64.7 cell lysates.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab109255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/1000.
IHC-Fr 1/100.
ICC/IF 1/500.

靶标

  • 功能
    Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) in IFN promoters and in the Q promoter (Qp) of EBV nuclear antigen 1 (EBNA1). Functions as a molecular switch for antiviral activity. Activated by phosphorylation in response to infection. Activation leads to nuclear retention, DNA binding, and derepression of transactivation ability.
  • 组织特异性
    Expressed predominantly in spleen, thymus and peripheral blood leukocytes.
  • 序列相似性
    Belongs to the IRF family.
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
  • 翻译后修饰
    In response to a viral infection, phosphorylated on the C-terminal serine cluster. Phosphorylation, and subsequent activation is inhibited by vaccinia virus protein E3.
    TRAF6-mediated ubiquitination is required for IRF7 activation.
  • 细胞定位
    Nucleus. Cytoplasm. The phosphorylated and active form accumulates selectively in the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • IMD39 antibody
    • Interferon regulatory factor 7 antibody
    • Interferon regulatory factor 7H antibody
    • IRF 7 antibody
    • IRF 7A antibody
    • IRF 7H antibody
    • IRF-7 antibody
    • IRF7 antibody
    • IRF7_HUMAN antibody
    • IRF7A antibody
    • IRF7B antibody
    • IRF7C antibody
    • IRF7H antibody
    see all

Anti-IRF7 antibody [EPR4718] 图像

  • All lanes : Anti-IRF7 antibody [EPR4718] (ab109255) at 1/5000 dilution (purified)

    Lane 1 : Rat spleen lysate
    Lane 2 : C6 whole cell lysate
    Lane 3 : Raw 264.7 lysate
    Lane 4 : PC-12 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size : 54 kDa
    Observed band size : 50,60 kDa (why is the actual band size different from the predicted?)

    Multiple bands observed are due to post-translational modifications such as acetylation, phosphorylation, and cross-linking.

  • Immunohistochemical staining of paraffin embedded human endometrial adenocarcinoma with purified ab109255 at a working dilution of 1 in 1000. The endometrial carcinoma cells show strong cytoplasmic staining. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunofluorescence staining of Jurkat cells showing cytoplasmic staining with purified ab109255 at a working dilution of 1 in 500, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1 in 400. ab7291, a mouse anti-tubulin antibody, was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab109255 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

  • Overlay histogram showing HuT cells stained with purified ab109255 (red line) at a dilution of 1/400. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was FITC goat anti-rabbit IgG at a dilution of 1/150. Isotype control antibody (black line) was rabbit IgG (monoclonal). Unlabelled sample (blue line) was also used as a control.

  • ab109255 (purified) at 1/120 immunoprecipitating IRF7 in Jurkat whol cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Anti-IRF7 antibody [EPR4718] (ab109255) at 1/5000 dilution (purified) + Human thymus lysate at 10 µg

    Secondary
    Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size : 54 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical staining of paraffin embedded human tonsil with purified ab109255 at a working dilution of 1 in 1000. The macrophage cells on the tonsil tissues show strong staining. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • All lanes : Anti-IRF7 antibody [EPR4718] (ab109255) at 1/1000 dilution (purified)

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg/ml per lane.

    Secondary
    Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1000

    Predicted band size : 54 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemical staining of paraffin embedded mouse liver with purified ab109255 at a working dilution of 1 in 1000. The Kupffer cells of mouse liver tissue show strong staining. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • All lanes : Anti-IRF7 antibody [EPR4718] (ab109255) at 1/10000 dilution (purified)

    Lane 1 : Jurkat whole cell lysate
    Lane 2 : Raji whole cell lysate
    Lane 3 : HUT-78 whole cell lysate
    Lane 4 : HEK293 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP goat anti-rabbit IgG (H + L) at 1/1000 dilution

    Predicted band size : 54 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

    Multiple bands observed are due to post-translational modifications such as acetylation, phosphorylation, and cross-linking.

     

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • ab109255 was used to detect IRF7 in Human tonsil tissue using Immunohistochemistry (Frozen sections). Human tonsil tissue was frozen and fixed with acetone fixed, sample permeabilized with 0.05% Tween and blocked with 6% BSA for 1 hr at room temp. Primary antibody was diluted 1/100 in 2% BSA in TBS/Tween and incubated for 12 hr at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal(1/25) was used as the secondary antibody. IRF7 staining in green, DAPI in blue.

    See Abreview

Anti-IRF7 antibody [EPR4718] (ab109255)参考文献

This product has been referenced in:
  • Pinilla-Vera M  et al. Full Spectrum of LPS Activation in Alveolar Macrophages of Healthy Volunteers by Whole Transcriptomic Profiling. PLoS One 11:e0159329 (2016). Read more (PubMed: 27434537) »
  • Patin EC  et al. IL-27 Induced by Select Candida spp. via TLR7/NOD2 Signaling and IFN-ß Production Inhibits Fungal Clearance. J Immunol 197:208-21 (2016). Read more (PubMed: 27259855) »

See all 10 Publications for this product

Product Wall

Application
IHC - Wholemount
Sample
Mouse Tissue (embryonic membranes)
Specification
embryonic membranes
Username

Mrs. Ira Rostovsky

Verified customer

提交于 Jun 26 2015

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 22°C
Sample
Mouse Tissue sections (whole mouse embryo sections)
Specification
whole mouse embryo sections
Permeabilization
Yes - 0.1% triton x100 in TBS
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 27 2014

Thank you for contacting us.

Yes, ab109255 can be used to detect endogenous mouse IRF7. We have tested it in WB, IP, Flow Cyt, and IHC-Fr. We have not yet tested it in immunocytochemistry (ICC/IF).

I hope this information is helpf...

Read More

Thank you for contacting us.

Regarding ab109255, the immunogen is within aa 200-250 and is able to detect isoforms A, B and D.


For ab62505:

The immunogen does not span a phosphorylated region (it is N-terminal of the pho...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Spleen whole extract)
Loading amount
100 µg
Specification
Spleen whole extract
Gel Running Conditions
Reduced Denaturing (Tris-Glycine -10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 30 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse spleen whole extract)
Loading amount
100 µg
Specification
Mouse spleen whole extract
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 30 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (tonsil)
Specification
tonsil
Fixative
Acetone
Permeabilization
Yes - 0.05% Tween
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (pDC)
Specification
pDC
Preparation
Cell harvesting/tissue preparation method: Ficoll
Sample buffer: PBS
Fixation
Paraformaldehyde
Permeabilization
Yes - saponin
Gating Strategy
FSC/SSC lymphocytes and then FSC-A/FSC-H singlets
Username

Abcam user community

Verified customer

提交于 Oct 04 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (splenocytes)
Loading amount
1e+006 cells
Specification
splenocytes
Treatment
Sendai Virus
Gel Running Conditions
Reduced Denaturing (4-12)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Marcella Flores

Verified customer

提交于 Sep 07 2012

Thank you for contacting us.

The IRF7 protein according to Uniprot (http://www.uniprot.org/uniprot/Q92985) have isoform molecular weight 54, 51, 18 and 55 kDa. Please note, the molecular weight for unprocessed protein (predicted) is based on...

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