Anti-IRF6抗体[2A12] (ab123880)

概述

  • 产品名称Anti-IRF6抗体[2A12]
    参阅全部 IRF6 一抗
  • 描述
    小鼠单克隆抗体[2A12] to IRF6
  • 经测试应用适用于: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length Human IRF6 protein (NP_006138) produced in HEK293 cell.

  • 阳性对照
    • 购买相配的WB阳性对照Recombinant Human IRF6 protein
    • WB: HEK293T cells transfected with pCMV6-ENTRY IRF6 cDNA IHC-P: adenocarcinoma of Human colon tissue, Human kidney tissue, carcinoma of Human lung tissue, adenocarcinoma of Human endometrium tissue ICC/IF: COS7 cells transiently transfected by pCMV6-ENTRY IRF6 Flow Cyt: HEK293T cells transfected with pCMV6-ENTRY IRF6 overexpress plasmid
  • 常规说明Dilute in PBS (pH 7.3) before use.

性能

应用

Our Abpromise guarantee covers the use of ab123880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Predicted molecular weight: 53 kDa.
IHC-P 1/150.
Flow Cyt 1/100. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

靶标

  • 功能Probable DNA-binding transcriptional activator. Key determinant of the keratinocyte proliferation-differentiation switch involved in appropriate epidermal development (By similarity). Plays a role in regulating mammary epithelial cell proliferation.
  • 组织特异性Expressed in normal mammary epithelial cells. Expression is reduced or absent in breast carcinomas.
  • 疾病相关Defects in IRF6 are a cause of van der Woude syndrome (VWS) [MIM:119300]; also known as lip-pit syndrome (LPS). It is an autosomal dominant developmental disorder characterized by lower lip pits, cleft lip and/or cleft palate. Penetrance is incomplete. Van der Woude and popliteal pterygium syndrome are allelic disorders.
    Defects in IRF6 are the cause of popliteal pterygium syndrome (PPS) [MIM:119500]. PPS is an autosomal dominant developmental disorder characterized by cleft lip and/or cleft palate, and skin and genital anomalies. Penetrance is incomplete and expressivity is variable. It shows orofacial phenotypic similarities with van der Woude syndrome. Van der Woude and popliteal pterygium syndrome are allelic disorders.
    Genetic variation in IRF6 is associated with non-syndromic orofacial cleft type 6 (OFC6) [MIM:608864]; also called non-syndromic cleft lip with or without cleft palate 6. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum.
  • 序列相似性Belongs to the IRF family.
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
  • 翻译后修饰Phosphorylated. Phosphorylation status depends on the cell cycle and is a signal for ubiquitination and proteasome-mediated degradation.
  • 细胞定位Nucleus. Cytoplasm. Translocates to nucleus in response to an activating signal.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Interferon regulatory factor 6 antibody
    • IRF 6 antibody
    • IRF-6 antibody
    • IRF6 antibody
    • IRF6_HUMAN antibody
    • LPS antibody
    • OFC 6 antibody
    • OFC6 antibody
    • OTTHUMP00000034677 antibody
    • OTTHUMP00000034678 antibody
    • PIT antibody
    • PPS antibody
    • PPS1 antibody
    • VWS antibody
    • VWS1 antibody
    see all

Anti-IRF6 antibody [2A12] 图像

  • All lanes : Anti-IRF6 antibody [2A12] (ab123880) at 1/2000 dilution

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY IRF6 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 53 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC201579)
  • ab123880, at a 1/150 dilution, staining IRF6 in paraffin embedded adenocarcinoma of Human colon tissue by Immunohistochemistry.
  • ab123880, at a 1/150 dilution, staining IRF6 in paraffin embedded Human kidney tissue by Immunohistochemistry.
  • ab123880, at a 1/150 dilution, staining IRF6 in paraffin embedded carcinoma of Human lung tissue by Immunohistochemistry.
  • ab123880, at a 1/150 dilution, staining IRF6 in paraffin embedded adenocarcinoma of Human endometrium tissue by Immunohistochemistry.
  • ab123880, at a 1/100 dilution, staining IRF6 in COS7 cells transiently transfected using pCMV6-ENTRY IRF6, by Immunofluorescence.
  • ab123880, at a 1/100 dilution, staining IRF6 in HEK293T cells transfected with either pCMV6-ENTRY IRF6 overexpress plasmid (Red), or empty vector control plasmid (Blue), by Flow Cytometry.

Anti-IRF6 antibody [2A12] (ab123880)参考文献

ab123880 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Granular Cell Layer)
Specification Granular Cell Layer
Fixative Formaldehyde
Permeabilization Yes - 0.5% Triton X-100
Blocking step Serum, plus monovalent Fab Fragment as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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提交于 May 18 2012

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