Anti-IRF5抗体[EPR6094] (ab124792)


  • 产品名称Anti-IRF5抗体[EPR6094]
    参阅全部 IRF5 一抗
  • 描述
    兔单克隆抗体[EPR6094] to IRF5
  • 经测试应用适用于: IHC-Fr, WB, Flow Cytmore details
    不适用于: ICC/IF,IHC-P or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human IRF5 (Q13568).

  • 阳性对照
    • U937, Ramos and THP-1 cell lysates, U937 cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-IRF5 antibody (Alexa Fluor® 488) [EPR6094] (ab193245)

    Anti-IRF5 antibody (Alexa Fluor® 647) [EPR6094] (ab192983)



Our Abpromise guarantee covers the use of ab124792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa).Can be blocked with IRF5 peptide (ab174256).
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for ICC/IF,IHC-P or IP.
  • 靶标

    • 疾病相关Genetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) [MIM:612245]. IBD14 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
      Genetic variations in IRF5 are associated with susceptibility to systemic lupus erythematosus type 10 (SLEB10) [MIM:612251]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
      Genetic variations in IRF5 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures.
    • 序列相似性Belongs to the IRF family.
      Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Interferon regulatory factor 5 antibody
      • Interferon regulatory factor 5 bone marrow variant antibody
      • IRF 5 antibody
      • IRF-5 antibody
      • Irf5 antibody
      • IRF5_HUMAN antibody
      • SLEB10 antibody
      see all

    Anti-IRF5 antibody [EPR6094] 图像

    • All lanes : Anti-IRF5 antibody [EPR6094] (ab124792) at 1/1000 dilution

      Lane 1 : U937 cell lysates
      Lane 2 : Ramos cell lysates
      Lane 3 : THP-1 cell lysates

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 56 kDa
    • Flow cytometric analysis of permeabilized U937 cells using ab124792 at 1/100 dilution (red) or a rabbit IgG (negative) (green).
    • Flow cytometry analysis of Plasmacytoid dendritic cells, staining IRF5 with ab124792.

      Cells were fixed in paraformaldehyde and fixed in saponin. The sample was incubated with the primary antibody (1/100 in 2% human serum + 0.5 mM EDTA) for 20 minutes at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/100) was used as the secondary antibody.

      Gating Strategy: FSC/SSC lymphocytes and then FSC-A/FSC-H singlets

      See Abreview

    Anti-IRF5 antibody [EPR6094] (ab124792)参考文献

    ab124792 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Human Cell (pDC)
    Specification pDC
    Preparation Cell harvesting/tissue preparation method: Ficoll
    Sample buffer: PBS
    Fixation Paraformaldehyde
    Permeabilization Yes - saponin
    Gating Strategy FSC/SSC lymphocytes and then FSC-A/FSC-H singlets

    Abcam user community

    Verified customer

    提交于 Oct 04 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Human Tissue sections (tonsil)
    Specification tonsil
    Fixative Acetone
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C

    Abcam user community

    Verified customer

    提交于 Oct 02 2012