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A synthetic peptide corresponding to 14 amino acids near the carboxyterminus of IRF3 (Human)
Our Abpromise guarantee covers the use of ab25950 in the following tested applications.
|IHC-P||Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|ELISA||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 47 kDa).Can be blocked with Human IRF3 peptide (ab39792).|
ab25950 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab25950 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab25950 at 2µg/ml staining IRF3 in mouse kidney by IHC.