Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 450-500 of Human IRF2BP1 (NP_056464.1).
HeLa, Jurkat and 293T whole cell lysates
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 62 kDa.
Use at 2-10 µg/mg of lysate.
Acts as a transcriptional repressor. Acts as a transcriptional corepressor in a IRF2-dependent manner. This repression is not mediated at least in part by histone deacetylase activities.
Belongs to the IRF2BP family.
The C-terminal RING-type zinc finger domain is necessary for interaction with BAP1.
Information by UniProt
Interferon regulatory factor 2 binding protein 1 antibody
Western blot - Anti-IRF2BP1 antibody (ab114970)
All lanes : Anti-IRF2BP1 antibody (ab114970) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : Jurkat whole cell lysate at 50 µg Lane 4 : 293T whole cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 62 kDa Exposure time: 3 minutes
Immunoprecipitation - Anti-IRF2BP1 antibody (ab114970)
Detection of IRF2BP1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab114970 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
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