概述

  • 产品名称Anti-IRF2抗体
    参阅全部 IRF2 一抗
  • 描述
    山羊多克隆抗体to IRF2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide:

    KTSDITQARVKSC

    , corresponding to C terminal amino acids 337-349 of Human IRF2.

  • 阳性对照
  • 常规说明Principal Names – IRF2; IRF-2; interferon regulatory factor 2 Official Gene Symbol - IRF2 GenBank Accession Number – NP_002190 LocusLink ID - 3660 (human); 16363 (mouse) Gene Ontology terms - RNA polymerase II transcription factor activity; cell proliferation; oncogenesis; negative regulation of transcription from Pol II promoter

性能

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应用

Our Abpromise guarantee covers the use of ab3388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 38 kDa).Can be blocked with Human IRF2 peptide (ab22943). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Li et al, Invest Ophthalmol Vis Sci. 1999 Apr;40(5):976-82.; PMID: 10102295.

靶标

  • 功能Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation.
  • 组织特异性Expressed throughout the epithelium of the colon. Also expressed in lamina propria.
  • 序列相似性Belongs to the IRF family.
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
  • 翻译后修饰Acetylated by CBP/ p300 during cell-growth. Acetylation on Lys-75 is required for stimulation of H4 promoter activity.
    The major sites of sumoylation are Lys-137 and Lys-293. Sumoylation by SUMO1 increases its transcriptional repressor activity on IRF1 and diminishes its ability to activate ISRE and H4 promoter.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp686F0244 antibody
    • Interferon regulatory factor 2 antibody
    • IRF 2 antibody
    • IRF-2 antibody
    • IRF2 antibody
    • IRF2_HUMAN antibody
    see all

Anti-IRF2 antibody 图像



  • Predicted band size : 38 kDa


    ab3388 staining (2µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

    The observed molecular weight corresponds to earlier findings in literature with different antibodies (Li et al, Invest Ophthalmol Vis Sci. 1999 Apr;40(5):976-82.; PMID: 10102295.

Anti-IRF2 antibody (ab3388)参考文献

This product has been referenced in:
  • Liljeroos M  et al. Bacterial ligand of TLR2 signals Stat activation via induction of IRF1/2 and interferon-alpha production. Cell Signal 20:1873-81 (2008). Read more (PubMed: 18652890) »

See 1 Publication for this product

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Thank you for your enquiry. All the information that we have about this antibody is contained on the antibody datasheet. To clarify this antibody has not been tested during developmental stages. This antibody is a mixture of many different goat...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"