概述

  • 产品名称Anti-IREB2抗体
    参阅全部 IREB2 一抗
  • 描述
    兔多克隆抗体to IREB2
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cat, Dog, Pig, Caenorhabditis elegans, Zebrafish
  • 免疫原

    Synthetic peptide: QVIQINLNSI VPSVSGPKRP QDRVAVTDMK SDFQACLNEK VGFKGFQIAA , corresponding to amino acids 432-481 of Human IREB2

  • 阳性对照
    • Fetal liver lysate

性能

应用

Our Abpromise guarantee covers the use of ab80339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 105 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

靶标

  • 功能RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.
  • 序列相似性Belongs to the aconitase/IPM isomerase family.
  • 翻译后修饰Ubiquitinated and degraded by the proteasome in presence of high level of iron and oxygen. Ubiquitinated by a SCF complex containing FBXL5. Upon iron and oxygen depletion FBXL5 is degraded, preventing ubiquitination and allowing its RNA-binding activity.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACO3 antibody
    • FLJ23381 antibody
    • IRE BP2 antibody
    • IRE-BP 2 antibody
    • IREB 2 antibody
    • IREB2 antibody
    • IREB2_HUMAN antibody
    • Iron regulatory protein 2 antibody
    • iron responsive element binding protein 2 antibody
    • Iron-responsive element-binding protein 2 antibody
    • IRP 2 antibody
    • IRP2 antibody
    • IRP2AD antibody
    • OTTHUMP00000185022 antibody
    see all

Anti-IREB2 antibody 图像

  • Anti-IREB2 antibody (ab80339) at 1 µg/ml + Fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 105 kDa
    Observed band size : 105 kDa

Anti-IREB2 antibody (ab80339)参考文献

ab80339 has not yet been referenced specifically in any publications.

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