概述

  • 产品名称
  • 描述
    山羊多克隆抗体to IREB2
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Pig, Orangutan
  • 免疫原

    Synthetic peptide:

    C-SIHYEGSEYKLSHGS

    , corresponding to internal sequence amino acids 489-503 of Human IREB2 (NP_004127.1).

  • 阳性对照
    • Human Liver lysate Human kidney

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab106926 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab106926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 3 - 6 µg/ml.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 105 kDa).

靶标

  • 功能
    RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.
  • 序列相似性
    Belongs to the aconitase/IPM isomerase family.
  • 翻译后修饰
    Ubiquitinated and degraded by the proteasome in presence of high level of iron and oxygen. Ubiquitinated by a SCF complex containing FBXL5. Upon iron and oxygen depletion FBXL5 is degraded, preventing ubiquitination and allowing its RNA-binding activity.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACO3 antibody
    • FLJ23381 antibody
    • IRE BP2 antibody
    • IRE-BP 2 antibody
    • IREB 2 antibody
    • IREB2 antibody
    • IREB2_HUMAN antibody
    • Iron regulatory protein 2 antibody
    • iron responsive element binding protein 2 antibody
    • Iron-responsive element-binding protein 2 antibody
    • IRP 2 antibody
    • IRP2 antibody
    • IRP2AD antibody
    • OTTHUMP00000185022 antibody
    see all

图片

  • All lanes : Anti-IREB2 antibody (ab106926) at 0.3 µg/ml

    Lane 1 : Human Liver lysate in RIPA buffer
    Lane 2 : Human Liver lysate in RIPA buffer with immunizing peptide

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique

    Predicted band size : 105 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • ab106926 at 3.8 µg/ml staining IREB2 in Human spleen by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed. Antibody was revealed with AP-staining.

文献

This product has been referenced in:
  • Cloonan SM  et al. Mitochondrial iron chelation ameliorates cigarette smoke-induced bronchitis and emphysema in mice. Nat Med 22:163-74 (2016). Read more (PubMed: 26752519) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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