Anti-IRE1 (phospho S724)抗体(Biotin) (ab81936)

概述

  • 产品名称
    Anti-IRE1 (phospho S724)抗体(Biotin)
    参阅全部 IRE1 一抗
  • 描述
    兔多克隆抗体to IRE1 (phospho S724) (Biotin)
  • 偶联物
    Biotin
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    不与反应: Monkey
  • 免疫原

    Synthetic peptide surrounding the phosphorylated serine 724 of human IRE1.

  • 阳性对照
    • Glucose treated pancreatic islets and endogenous protein of pancreatic beta cells and insuli. COS-7 expressing wild-type IRE1. Min6 cells treated with glucose.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: None
    Constituents: 30% Glycerol, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab81936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).

靶标

  • 功能
    Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
  • 组织特异性
    Ubiquitously expressed. High levels observed in pancreatic tissue.
  • 序列相似性
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 KEN domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Autophosphorylated.
  • 细胞定位
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
    • Endoplasmic reticulum to nucleus signaling 1 antibody
    • Endoplasmic reticulum-to-nucleus signaling 1 antibody
    • Endoribonuclease antibody
    • ER to nucleus signaling 1 antibody
    • ERN 1 antibody
    • Ern1 antibody
    • ERN1_HUMAN antibody
    • hIRE 1p antibody
    • hIRE1p antibody
    • Inositol requiring 1 antibody
    • Inositol requiring 1, S. cerevisiae, homolog of antibody
    • Inositol requiring enzyme 1, S. cerevisiae, homolog of antibody
    • Inositol requiring protein 1 antibody
    • inositol-requiring enzyme 1 antibody
    • Inositol-requiring protein 1 antibody
    • IRE 1 antibody
    • IRE 1a antibody
    • IRE 1P antibody
    • Ire1 alpha antibody
    • Ire1-alpha antibody
    • IRE1a antibody
    • Ire1alpha antibody
    • IRE1P antibody
    • MGC163277 antibody
    • MGC163279 antibody
    • Protein kinase/endoribonuclease antibody
    • RGD1559716 antibody
    • Serine/threonine protein kinase/endoribonuclease IRE1 antibody
    see all

实验方案

Anti-IRE1 (phospho S724) antibody (Biotin) (ab81936)参考文献

ab81936 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (AR42J)
Permeabilization
Yes - 10% Triton x100
Specification
AR42J
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Fixative
Formaldehyde
Username

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提交于 Jul 24 2017

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