概述

  • 产品名称Anti-IRE1抗体
    参阅全部 IRE1 一抗
  • 描述
    小鼠单克隆抗体to IRE1
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: EEVINLVDQT SENAPTTVSR DVEEKPAHAP ARPEAPVDSM LKDMATIILS TFLLIGWVAF IITYPLSMHQ QQQLQHQQFQ KELEKIQLLQ QQQQQLPFHP , corresponding to amino acids 401-500 of Human IRE1 (AAC25991) with a 26 kDa tag

  • 阳性对照
    • HeLa cell lysate, Human esophagus tissue

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab87964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
IHC-P
  • 应用说明ELISA: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 1.5 µg/ml. Antigen retrieval is not essential but may optimise staining when the microwave method is used.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 110 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
    • 组织特异性Ubiquitously expressed. High levels observed in pancreatic tissue.
    • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
      Contains 1 KEN domain.
      Contains 1 protein kinase domain.
    • 翻译后修饰Autophosphorylated.
    • 细胞定位Endoplasmic reticulum membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
      • Endoplasmic reticulum to nucleus signaling 1 antibody
      • Endoplasmic reticulum-to-nucleus signaling 1 antibody
      • Endoribonuclease antibody
      • ER to nucleus signaling 1 antibody
      • ERN 1 antibody
      • Ern1 antibody
      • ERN1_HUMAN antibody
      • hIRE 1p antibody
      • hIRE1p antibody
      • Inositol requiring 1 antibody
      • Inositol requiring 1, S. cerevisiae, homolog of antibody
      • Inositol requiring enzyme 1, S. cerevisiae, homolog of antibody
      • Inositol requiring protein 1 antibody
      • inositol-requiring enzyme 1 antibody
      • Inositol-requiring protein 1 antibody
      • IRE 1 antibody
      • IRE 1a antibody
      • IRE 1P antibody
      • Ire1 alpha antibody
      • Ire1-alpha antibody
      • IRE1a antibody
      • Ire1alpha antibody
      • IRE1P antibody
      • MGC163277 antibody
      • MGC163279 antibody
      • Protein kinase/endoribonuclease antibody
      • RGD1559716 antibody
      • Serine/threonine protein kinase/endoribonuclease IRE1 antibody
      see all

    Anti-IRE1 antibody 图像

    • Anti-IRE1 antibody (ab87964) at 1 µg/ml + Immunogen (Recombinant protein) at 0.2 µg
      Developed using the ECL technique

      Predicted band size : 110 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    • Anti-IRE1 antibody (ab87964) at 1 µg/ml + HeLa cell lysate at 50 µg
      Developed using the ECL technique

      Predicted band size : 110 kDa
    • ab87964 at 1.5µg/ml staining IRE1 in Human esophagus by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.

    Anti-IRE1 antibody (ab87964)参考文献

    ab87964 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"