概述

  • 产品名称Anti-IRE1抗体
    参阅全部 IRE1 一抗
  • 描述
    兔多克隆抗体to IRE1
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide consisting of 16 amino acid from near the carboxy terminus (Human).

  • 阳性对照
    • WB: A20 cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab37073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 107 kDa (predicted molecular weight: 110 kDa).
Please note there is a smaller isoform of IRE1 in rat (predicted molecular weight:68.6kDa; accession number is EDM06401). Block for 2 hours at RT or overnight at 4C using 5% non-fat milk.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
  • 组织特异性Ubiquitously expressed. High levels observed in pancreatic tissue.
  • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 KEN domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated.
  • 细胞定位Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
    • Endoplasmic reticulum to nucleus signaling 1 antibody
    • Endoplasmic reticulum-to-nucleus signaling 1 antibody
    • Endoribonuclease antibody
    • ER to nucleus signaling 1 antibody
    • ERN 1 antibody
    • Ern1 antibody
    • ERN1_HUMAN antibody
    • hIRE 1p antibody
    • hIRE1p antibody
    • Inositol requiring 1 antibody
    • Inositol requiring 1, S. cerevisiae, homolog of antibody
    • Inositol requiring enzyme 1, S. cerevisiae, homolog of antibody
    • Inositol requiring protein 1 antibody
    • inositol-requiring enzyme 1 antibody
    • Inositol-requiring protein 1 antibody
    • IRE 1 antibody
    • IRE 1a antibody
    • IRE 1P antibody
    • Ire1 alpha antibody
    • Ire1-alpha antibody
    • IRE1a antibody
    • Ire1alpha antibody
    • IRE1P antibody
    • MGC163277 antibody
    • MGC163279 antibody
    • Protein kinase/endoribonuclease antibody
    • RGD1559716 antibody
    • Serine/threonine protein kinase/endoribonuclease IRE1 antibody
    see all

Anti-IRE1 antibody 图像

  • Lane 1 : Anti-IRE1 antibody (ab37073) at 0.5 µg/ml
    Lane 2 : Anti-IRE1 antibody (ab37073) at 1 µg/ml
    Lane 3 : Anti-IRE1 antibody (ab37073) at 2 µg/ml

    Lane 1 : A20 cell lysate
    Lane 2 : A20 cell lysate
    Lane 3 : A20 cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    goat anti-rabbit HRP at 1/10000 dilution

    Predicted band size : 110 kDa
    Observed band size : 107 kDa (why is the actual band size different from the predicted?)
  • ab37073, at 1 µg/ml, staining IRE1 in A20 cells with by ICC/IF.
  • Immunofluorescence of IRE1p in A20 cells using ab37073 at 2 ug/ml.

Anti-IRE1 antibody (ab37073)参考文献

This product has been referenced in:
  • Son SM  et al. Reduced IRE1a mediates apoptotic cell death by disrupting calcium homeostasis via the InsP3 receptor. Cell Death Dis 5:e1188 (2014). WB ; Human . Read more (PubMed: 24743743) »
  • Wang Y  et al. Inhibition of JNK by novel curcumin analog C66 prevents diabetic cardiomyopathy with a preservation of cardiac metallothionein expression. Am J Physiol Endocrinol Metab 306:E1239-47 (2014). Mouse . Read more (PubMed: 24714399) »

See all 10 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (CEC)
Loading amount 30 µg
Specification CEC
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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提交于 Apr 22 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"