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Our Abpromise guarantee covers the use of ab37073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 107 kDa (predicted molecular weight: 110 kDa).
Please note there is a smaller isoform of IRE1 in rat (predicted molecular weight:68.6kDa; accession number is EDM06401). Block for 2 hours at RT or overnight at 4C using 5% non-fat milk.
|ICC/IF||Use a concentration of 1 µg/ml.|
|ICC||Use a concentration of 1 µg/ml.|
ab37073, at 1 µg/ml, staining IRE1 in A20 cells with by ICC.
Immunocytochemistry/Immunofluorescence of IRE1p in A20 cells using ab37073 at 2 ug/ml.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"