Anti-IRE1抗体[9F2] (ab96481)
Key features and details
- Mouse monoclonal [9F2] to IRE1
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-IRE1抗体[9F2]
参阅全部 IRE1 一抗 -
描述
小鼠单克隆抗体[9F2] to IRE1 -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Purified recombinant fragment of Human IRE1 (aa282-433) expressed in E.coli.
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阳性对照
- Raji, A431, Jurkat, Hela and HEK293 cell lysate; Human brain and stomach tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
9F2 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96481于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (3) |
1/500 - 1/2000. Predicted molecular weight: 110 kDa.
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IHC-P | (1) |
1/200 - 1/1000.
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Flow Cyt |
1/100 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
1/500 - 1/2000. Predicted molecular weight: 110 kDa. |
IHC-P
1/200 - 1/1000. |
Flow Cyt
1/100 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. -
组织特异性
Ubiquitously expressed. High levels observed in pancreatic tissue. -
序列相似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 KEN domain.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated. -
细胞定位
Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 10595 Human
- Entrez Gene: 2081 Human
- Omim: 604033 Human
- SwissProt: O75460 Human
- SwissProt: Q76MJ5 Human
- Unigene: 133982 Human
- Unigene: 592041 Human
- Unigene: 700027 Human
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别名
- Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
- Endoplasmic reticulum to nucleus signaling 1 antibody
- Endoplasmic reticulum-to-nucleus signaling 1 antibody
see all
图片
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All lanes : Anti-IRE1 antibody [9F2] (ab96481) at 1/500 dilution
Lane 1 : Raji cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : HEK293 cell lysate
Predicted band size: 110 kDa -
Immunohistochemical analysis of paraffin-embedded Human brain tissue (A) and stomach tissue (B), showing cytoplasmic localization using ab96481 at 1/200 dilution with DAB staining.
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Overlay histogram showing Raji cells stained with ab96481 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab96481, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Raji cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab96481 被引用在 9 文献中.
- Aghaei M et al. The Role of BiP and the IRE1α-XBP1 Axis in Rhabdomyosarcoma Pathology. Cancers (Basel) 13:N/A (2021). PubMed: 34638414
- Nie J et al. Activation of CaMKII via ER-stress mediates coxsackievirus B3-induced cardiomyocyte apoptosis. Cell Biol Int 44:488-498 (2020). PubMed: 31631456
- Reich S et al. A multi-omics analysis reveals the unfolded protein response regulon and stress-induced resistance to folate-based antimetabolites. Nat Commun 11:2936 (2020). PubMed: 32522993
- Wu Y et al. Dual role for inositol-requiring enzyme 1a in promoting the development of hepatocellular carcinoma during diet-induced obesity in mice. Hepatology 68:533-546 (2018). PubMed: 29506314
- Li K et al. Deficiency of the IRE1a-Autophagy Axis Enhances the Antitumor Effects of the Oncolytic Virus M1. J Virol 92:N/A (2018). PubMed: 29263275
- Jeon YJ et al. miRNA-mediated TUSC3 deficiency enhances UPR and ERAD to promote metastatic potential of NSCLC. Nat Commun 9:5110 (2018). PubMed: 30504895
- Ohana J et al. dTCApFs, a derivative of a novel human hormone peptide, induces apoptosis in cancer cells through a mechanism involving loss of Golgi function. Mol Clin Oncol 7:991-999 (2017). PubMed: 29285362
- Wang L et al. Gemcitabine treatment induces endoplasmic reticular (ER) stress and subsequently upregulates urokinase plasminogen activator (uPA) to block mitochondrial-dependent apoptosis in Panc-1 cancer stem-like cells (CSCs). PLoS One 12:e0184110 (2017). PubMed: 28854261
- Tamura K et al. SHISA2 enhances the aggressive phenotype in prostate cancer through the regulation of WNT5A expression. Oncol Lett 14:6650-6658 (2017). PubMed: 29344118