使用敲除细胞株进行验证RabMAb

Anti-IRAKM抗体[Y278] (ab32394)

概述

  • 产品名称Anti-IRAKM抗体[Y278]
    参阅全部 IRAKM 一抗
  • 描述
    兔单克隆抗体[Y278] to IRAKM
  • 经测试应用适用于: IP, WBmore details
    不适用于: Flow Cyt,ICC or IHC
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues in the C-term of human IRAK-3

  • 阳性对照
    • A431 cell lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab32394 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP 1/50.
WB 1/20000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
  • 应用说明Is unsuitable for Flow Cyt,ICC or IHC.
  • 靶标

    • 功能Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex.
    • 组织特异性Expressed predominantly in peripheral blood lymphocytes.
    • 疾病相关Defects in IRAK3 are associated with susceptibility to asthma-related traits type 5 (ASRT5) [MIM:611064]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing, dyspnea, bronchial hyperresponsiveness as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis.
    • 序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
      Contains 1 death domain.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ASRT5 antibody
      • IL 1 receptor associated kinase M antibody
      • IL-1 receptor-associated kinase M antibody
      • IL1 receptor associated kinase M antibody
      • Interleukin 1 receptor associated kinase 3 antibody
      • Interleukin 1 receptor associated kinase M antibody
      • Interleukin-1 receptor-associated kinase 3 antibody
      • IRAK M antibody
      • IRAK-3 antibody
      • IRAK-M antibody
      • Irak3 antibody
      • IRAK3_HUMAN antibody
      see all

    Anti-IRAKM antibody [Y278] 图像



    • Predicted band size : 68 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: IRAKM knockout HAP1 cell lysate (20 µg)

      Lanes 1 - 2: Merged signal (red and green). Green - ab32394 observed at 69 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab32394 was shown to specifically react with IRAKM when IRAKM knockout samples were used. Wild-type and IRAKM knockout samples were subjected to SDS-PAGE. ab32394 and ab8245 (loading control to GAPDH) diluted to 1/5000 and 1/10000 respectively were incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Anti-IRAKM antibody [Y278] (ab32394) at 1/20000 dilution + A431 cell lysate

      Predicted band size : 68 kDa
      Observed band size : 68 kDa

    Anti-IRAKM antibody [Y278] (ab32394)参考文献

    ab32394 has not yet been referenced specifically in any publications.

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