重组Anti-IRAKM抗体[Y278] (ab32394)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y278] to IRAKM
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-IRAKM抗体[Y278]
参阅全部 IRAKM 一抗 -
描述
兔单克隆抗体[Y278] to IRAKM -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC or ICC/IF -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human IRAKM aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q9Y616 -
阳性对照
- A431 cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
Y278 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32394于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/20000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
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说明 |
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WB
1/20000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). |
靶标
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功能
Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex. -
组织特异性
Expressed predominantly in peripheral blood lymphocytes. -
疾病相关
Defects in IRAK3 are associated with susceptibility to asthma-related traits type 5 (ASRT5) [MIM:611064]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing, dyspnea, bronchial hyperresponsiveness as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis. -
序列相似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 death domain.
Contains 1 protein kinase domain. - Information by UniProt
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数据库链接
- Entrez Gene: 11213 Human
- Omim: 604459 Human
- SwissProt: Q9Y616 Human
- Unigene: 369265 Human
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别名
- ASRT5 antibody
- IL 1 receptor associated kinase M antibody
- IL-1 receptor-associated kinase M antibody
see all
图片
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All lanes : Anti-IRAKM antibody [Y278] (ab32394) at 1/20000 dilution
Lane 1 : Wild-type THP-1 cell lysate
Lane 2 : IRAK3 knockout THP-1 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 68 kDaFalse colour image of Western blot: Anti-IRAKM antibody [Y278] staining at 1/20000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab32394 was shown to bind specifically to IRAKM. A band was observed at 68 kDa in wild-type THP-1 cell lysates with no signal observed at this size in IRAK3 knockout cell line ab281629 (knockout cell lysate ab282979). To generate this image, wild-type and IRAK3 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: IRAKM knockout HAP1 cell lysate (20 µg)Lanes 1 - 2: Merged signal (red and green). Green - ab32394 observed at 69 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32394 was shown to specifically react with IRAKM when IRAKM knockout samples were used. Wild-type and IRAKM knockout samples were subjected to SDS-PAGE. ab32394 and ab8245 (loading control to GAPDH) diluted to 1/5000 and 1/10000 respectively were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
Anti-IRAKM antibody [Y278] (ab32394) at 1/20000 dilution + A431 cell lysate
Predicted band size: 68 kDa
Observed band size: 68 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab32394 被引用在 2 文献中.
- Tiberti S et al. GZMKhigh CD8+ T effector memory cells are associated with CD15high neutrophil abundance in non-metastatic colorectal tumors and predict poor clinical outcome. Nat Commun 13:6752 (2022). PubMed: 36347862
- Wei W et al. IL-1 signaling pathway molecules as key markers in childhood asthma. Pediatr Allergy Immunol 32:305-313 (2021). PubMed: 33025692