Anti-IRAK4 (phospho T345)抗体[A8A8] (ab113656)

概述

  • 产品名称Anti-IRAK4 (phospho T345)抗体[A8A8]
    参阅全部 IRAK4 一抗
  • 描述
    小鼠单克隆抗体[A8A8] to IRAK4 (phospho T345)
  • 特异性ab113656 does not cross-react with non-phosphospecific peptide.
  • 经测试应用适用于: WB, IP, IHC-P, ELISA, Dot blot, Flow Cytmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Cow, Dog
  • 免疫原

    Synthetic peptide surrounding to the epitope -

    VMTSR

    - of Human IRAK4.

  • 阳性对照
    • Jurkat Cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab113656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 52 kDa).
IP Use a concentration of 2 - 5 µg/ml.
IHC-P Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.

Tested with immunizing peptide in peptide ELISA.

Dot blot 1/1000.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Required for the efficient recruitment of IRAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Phosphorylates IRAK1.
  • 疾病相关Defects in IRAK4 are the cause of recurrent isolated invasive pneumococcal disease type 1 (IPD1) [MIM:610799]. Recurrent invasive pneumococcal disease (IPD) is defined as two episodes of IPD occurring at least 1 month apart, whether caused by the same or different serotypes or strains. Recurrent IPD occurs in at least 2% of patients in most series, making IPD the most important known risk factor for subsequent IPD.
    Defects in IRAK4 are the cause of IRAK4 deficiency (IRAK4D) [MIM:607676]. IRAK4 deficiency causes extracellular pyogenic bacterial and fungal infections in otherwise healthy children.
  • 序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
    Contains 1 death domain.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • IL-1 receptor-associated kinase 4 antibody
    • Interleukin 1 receptor associated kinase 4 mutant form 1 antibody
    • Interleukin-1 receptor-associated kinase 4 antibody
    • Interleukin1 receptor associated kinase 4 antibody
    • IPD1 antibody
    • IRAK 4 antibody
    • IRAK-4 antibody
    • IRAK4 antibody
    • IRAK4 mutated form 1 antibody
    • IRAK4_HUMAN antibody
    • LOC 51135 antibody
    • NY REN 64 antibody
    • NY REN 64 antigen antibody
    • NY-REN-64 antibody
    • REN64 antibody
    • Renal carcinoma antigen NY-REN-64 antibody
    see all

Anti-IRAK4 (phospho T345) antibody [A8A8] 图像

  • All lanes : Anti-IRAK4 (phospho T345) antibody [A8A8] (ab113656) at 1/500 dilution

    Lane 1 : Jurkat cells stimulated by 50 ng/ml IL1 (10 min)
    Lane 2 : Jurkat cells stimulated by 50 ng/ml IL1 (10 min) with phospho specific peptide
    Lane 3 : Jurkat cells stimulated by 50 ng/ml IL1 (10 min) with non-phospho specific peptide


    Predicted band size : 52 kDa
  • Dot clot using ab113656 at 1/1000 dilution:
    A: 1µg IRAK4(pT345) peptide
    B: 1µg IRAK4(non phosphorylated) peptide
  • Overlay histogram showing K562 cells stained with ab113656 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab113656, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-IRAK4 (phospho T345) antibody [A8A8] (ab113656)参考文献

ab113656 has not yet been referenced specifically in any publications.

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