The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at a concentration of 10 µg/ml.
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 50 kDa. Can be blocked with IRAK4 peptide (448-460) (ab39782).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Required for the efficient recruitment of IRAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Phosphorylates IRAK1.
Defects in IRAK4 are the cause of recurrent isolated invasive pneumococcal disease type 1 (IPD1) [MIM:610799]. Recurrent invasive pneumococcal disease (IPD) is defined as two episodes of IPD occurring at least 1 month apart, whether caused by the same or different serotypes or strains. Recurrent IPD occurs in at least 2% of patients in most series, making IPD the most important known risk factor for subsequent IPD. Defects in IRAK4 are the cause of IRAK4 deficiency (IRAK4D) [MIM:607676]. IRAK4 deficiency causes extracellular pyogenic bacterial and fungal infections in otherwise healthy children.
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily. Contains 1 death domain. Contains 1 protein kinase domain.
Western blot analysis of IRAK4 in HeLa cell lysate with ab5985 at 1 (lane 1), 2 (lane 2), and 4 (lane 3) µg/ml, respectively. Western blot analysis of IRAK4 in HeLa cell lysate with ab5985 at 1 (lane 1), 2 (lane 2), and 4 (lane 3) µg/ml, respectively.
ab5985 at 10µg/ml staining IRAK-4 in K562 by ICC/IF
Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)This image was kindly supplied by Dr Mahesh Shivananjappa by Abreview
ab5985 at a 1/100 dilution staining IRAK4 in human PMN cells by Immunocytochemistry/ Immunofluorescence incubated for 4 hours at 37°C. Fixed with PFA. Blocked using 2% BSA for 1 hour at 22°C. Secondary used at 1/250 polyclonal Goat anti-rabbit conjugated to Alexa Fluor 568. Left image: DAPI staining nuclei (blue)Middle image: IRAK4 (red) Right image: Overlay