概述

  • 产品名称Anti-IRAK4抗体
    参阅全部 IRAK4 一抗
  • 描述
    兔多克隆抗体to IRAK4
  • 特异性This antibody is specific to IRAK4 and does not cross react with other IRAK proteins.
  • 经测试应用适用于: ICC, ICC/IF, WBmore details
  • 种属反应性
    与反应: Sheep, Human
  • 免疫原

    Synthetic peptide corresponding to amino acids at the carboxy terminus end of IRAK-4 (Human).

  • 阳性对照
    • HeLa whole cell lysate.
  • 常规说明Stable for one year.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab5985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC
ICC/IF
WB
  • 应用说明ICC/IF: Use at a concentration of 10 µg/ml.
    WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 50 kDa. Can be blocked with IRAK4 peptide (448-460) (ab39782).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Required for the efficient recruitment of IRAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Phosphorylates IRAK1.
    • 疾病相关Defects in IRAK4 are the cause of recurrent isolated invasive pneumococcal disease type 1 (IPD1) [MIM:610799]. Recurrent invasive pneumococcal disease (IPD) is defined as two episodes of IPD occurring at least 1 month apart, whether caused by the same or different serotypes or strains. Recurrent IPD occurs in at least 2% of patients in most series, making IPD the most important known risk factor for subsequent IPD.
      Defects in IRAK4 are the cause of IRAK4 deficiency (IRAK4D) [MIM:607676]. IRAK4 deficiency causes extracellular pyogenic bacterial and fungal infections in otherwise healthy children.
    • 序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
      Contains 1 death domain.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • 数据库链接
    • 别名
      • IL-1 receptor-associated kinase 4 antibody
      • Interleukin 1 receptor associated kinase 4 mutant form 1 antibody
      • Interleukin-1 receptor-associated kinase 4 antibody
      • Interleukin1 receptor associated kinase 4 antibody
      • IPD1 antibody
      • IRAK 4 antibody
      • IRAK-4 antibody
      • IRAK4 antibody
      • IRAK4 mutated form 1 antibody
      • IRAK4_HUMAN antibody
      • LOC 51135 antibody
      • NY REN 64 antibody
      • NY REN 64 antigen antibody
      • NY-REN-64 antibody
      • REN64 antibody
      • Renal carcinoma antigen NY-REN-64 antibody
      see all

    Anti-IRAK4 antibody 图像

    • Western blot analysis of IRAK4 in HeLa cell lysate with ab5985 at 1 (lane 1), 2 (lane 2), and 4 (lane 3) µg/ml, respectively.
    • ab5985 at 10µg/ml staining IRAK-4 in K562 by ICC/IF

    • ab5985 at a 1/100 dilution staining IRAK4 in human PMN cells by Immunocytochemistry/ Immunofluorescence incubated for 4 hours at 37°C. Fixed with PFA. Blocked using 2% BSA for 1 hour at 22°C. Secondary used at 1/250 polyclonal Goat anti-rabbit conjugated to Alexa Fluor 568.
      Left image: DAPI staining nuclei (blue)
      Middle image: IRAK4 (red)
      Right image: Overlay

      See Abreview

    • Immunofluorescence of IRAK-4 in K562 cells using ab5985 at 10 ug/ml.

    Anti-IRAK4 antibody (ab5985)参考文献

    This product has been referenced in:
    • Kallapur SG  et al. Pulmonary and systemic endotoxin tolerance in preterm fetal sheep exposed to chorioamnionitis. J Immunol 179:8491-9 (2007). WB ; Sheep . Read more (PubMed: 18056396) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry
    Sample Human Cell (PMN)
    Specification PMN
    Fixative Paraformaldehyde
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    提交于 Dec 29 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (MRC-5)
    Loading amount 1e+006 cells
    Specification MRC-5
    Blocking step Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    提交于 Oct 17 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"