概述

  • 产品名称Anti-IRAK2抗体
    参阅全部 IRAK2 一抗
  • 描述
    兔多克隆抗体to IRAK2
  • 特异性This antibody reacts with IRAK2, it does not react with IRAK
  • 经测试应用适用于: ICC/IF, ICC, IP, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A synthetic peptide corresponding to 17 amino acids near the C terminus of mouse IRAK2a, (which is common to all four isoforms).

  • 阳性对照
    • RAW2647 whole cell lysate and A20 cells

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab62419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
ICC
IP
WB
  • 应用说明ICC: Use at a concentration of 1 µg/ml.
    IP: Use at an assay dependent dilution (PMID 19224918).
    WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization.
    • 组织特异性Expressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes.
    • 序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
      Contains 1 death domain.
      Contains 1 protein kinase domain.
    • 结构域The protein kinase domain is predicted to be catalytically inactive.
    • Information by UniProt
    • 数据库链接
    • 别名
      • il1rak2 antibody
      • Interleukin 1 receptor associated kinase 2 antibody
      • Interleukin 1 receptor associated kinase like 2 antibody
      • Interleukin-1 receptor-associated kinase-like 2 antibody
      • IRAK 2 antibody
      • IRAK-2 antibody
      • Irak2 antibody
      • IRAK2_HUMAN antibody
      • MGC150550 antibody
      see all

    Anti-IRAK2 antibody 图像


    • Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 65 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)


      Exposure time : 15 seconds

      This image is courtesy of an anonymous abreview.

      Anti-IRAK antibody (ab62419) reactivity with reduced wildtype (WT) or IRAK2 knockout (KO) mouse embryonic fibroblast (MEF) cell lysate. After SDS-PAGE, membranes were blocked in 5% milk for 1h at 25°C before incubation with ab62419 (1µg/ml dilution in 5% milk in TBS/0.5% Tween) for 16h at 4ºC. The blot was then incubated with an anti-Rabbit HRP-conjugated secondary antibody before developing with ECL.

      See Abreview

    • Lane 1 : Anti-IRAK2 antibody (ab62419) at 0.5 µg/ml
      Lane 2 : Anti-IRAK2 antibody (ab62419) at 1 µg/ml
      Lane 3 : Anti-IRAK2 antibody (ab62419) at 2 µg/ml

      Lane 1 : RAW264.7 whole cell lysate
      Lane 2 : RAW264.7 whole cell lysate
      Lane 3 : RAW264.7 whole cell lysate

      Lysates/proteins at 15 µg per lane.


      Predicted band size : 65 kDa
      Observed band size : 65 kDa
    • Immunocytochemistry of IRAK2 in A-20 cells with ab62419 antibody at 1 µg/ml.
    • Immunofluorescence of IRAK2 in A20 cells using ab62419 at 10 ug/ml.

    Anti-IRAK2 antibody (ab62419)参考文献

    This product has been referenced in:
    • Martin BN  et al. IKKa negatively regulates ASC-dependent inflammasome activation. Nat Commun 5:4977 (2014). Read more (PubMed: 25266676) »
    • Xu H  et al. Notch-RBP-J signaling regulates the transcription factor IRF8 to promote inflammatory macrophage polarization. Nat Immunol 13:642-50 (2012). WB, IP . Read more (PubMed: 22610140) »

    See all 5 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (8%)
    Sample Mouse Cell lysate - whole cell (MEFs)
    Specification MEFs
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

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    Verified customer

    提交于 Sep 09 2013

    Application Immunoprecipitation
    Immuno-precipitation step Protein G
    Sample Human Cell lysate - whole cell (HEK293)
    Specification HEK293
    Total protein in input 500 µg
    Username

    Abcam user community

    Verified customer

    提交于 Sep 09 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"