Anti-IRAK2抗体(ab25957)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to IRAK2
  • 特异性
    ab25957 recognises IRAK2 but does not cross-react with IRAK.
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Synthetic peptide, corresponding to 16 C terminal amino acids of Mouse IRAK2a isoform. This sequence is common to all four isoforms.

  • 阳性对照
    • Whole cell lysate from A-20 cells.

性能

应用

Our Abpromise guarantee covers the use of ab25957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 65 kDa.

靶标

  • 功能
    Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization.
  • 组织特异性
    Expressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes.
  • 序列相似性
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
    Contains 1 death domain.
    Contains 1 protein kinase domain.
  • 结构域
    The protein kinase domain is predicted to be catalytically inactive.
  • Information by UniProt
  • 数据库链接
  • 别名
    • il1rak2 antibody
    • Interleukin 1 receptor associated kinase 2 antibody
    • Interleukin 1 receptor associated kinase like 2 antibody
    • Interleukin-1 receptor-associated kinase-like 2 antibody
    • IRAK 2 antibody
    • IRAK-2 antibody
    • Irak2 antibody
    • IRAK2_HUMAN antibody
    • MGC150550 antibody
    see all

图片

  • Lane 1 : Anti-IRAK2 antibody (ab25957) at 0.5 µg/ml
    Lane 2 : Anti-IRAK2 antibody (ab25957) at 1 µg/ml
    Lane 3 : Anti-IRAK2 antibody (ab25957) at 2 µg/ml

    Lane 1 : A-20 whole cell lysate
    Lane 2 : A-20 whole cell lysate
    Lane 3 : A-20 whole cell lysate


    Predicted band size : 65 kDa

实验方案

文献

ab25957 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (LIVER)
Specification
LIVER
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

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提交于 Nov 10 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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