概述

  • 产品名称Anti-IRAK抗体
    参阅全部 IRAK 一抗
  • 描述
    兔多克隆抗体to IRAK
  • 特异性ab62700 detects endogenous levels of total IRAK protein.
  • 经测试应用适用于: ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic non phosphopeptide derived from human IRAK around the phosphorylation site of threonine 209 (R-G-TP-H-N).

  • 阳性对照
    • Human breast carcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab62700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000.
IHC-P 1/50 - 1/100.

靶标

  • 功能Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1.
  • 组织特异性Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.
  • 序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated or is transphosphorylated by IRAK4 following recruitment to the IL-1RI. In the case of isoform 1, this is linked to ubiquitination and degradation.
    Polyubiquitinated; after cell stimulation with IL-1-beta. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Il1rak antibody
    • Interleukin 1 receptor associated kinase 1 antibody
    • Interleukin-1 receptor-associated kinase 1 antibody
    • IRAK antibody
    • IRAK-1 antibody
    • Irak1 antibody
    • IRAK1_HUMAN antibody
    • mPLK antibody
    • OTTHUMP00000026014 antibody
    • OTTHUMP00000026015 antibody
    • OTTHUMP00000026020 antibody
    • OTTHUMP00000180621 antibody
    • Pelle antibody
    • Pelle homolog antibody
    • Pelle-like protein kinase antibody
    • Plpk antibody
    see all

Anti-IRAK antibody 图像

  • ab62700, at 1/50 dilution, staining IRAK in paraffin embedded human breast carcinoma tissue by Immunohistochemistry in the absence or presence of the immunising peptide.

Anti-IRAK antibody (ab62700)参考文献

This product has been referenced in:
  • Ahmad R  et al. Increased expression of the interleukin-1 receptor-associated kinase (IRAK)-1 is associated with adipose tissue inflammatory state in obesity. Diabetol Metab Syndr 7:71 (2015). IHC-P ; Human . Read more (PubMed: 26312071) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab62700.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"