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Our Abpromise guarantee covers the use of ab55316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 77 kDa.|
|IHC-P||Use a concentration of 3 µg/ml.|
|ICC/IF||Use a concentration of 10 µg/ml.|
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: IRAK1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Hek293 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab55316 observed at 85 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab55316 was shown to specifically react with IRAK1 when IRAK1 knockout samples were used. Wild-type and IRAK1 knockout samples were subjected to SDS-PAGE. Ab55316 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
ab55316 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"