使用敲除细胞株进行验证

Anti-IRAK抗体(ab55316)

概述

性能

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应用

Our Abpromise guarantee covers the use of ab55316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 77 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1.
  • 组织特异性Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.
  • 序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated or is transphosphorylated by IRAK4 following recruitment to the IL-1RI. In the case of isoform 1, this is linked to ubiquitination and degradation.
    Polyubiquitinated; after cell stimulation with IL-1-beta. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Il1rak antibody
    • Interleukin 1 receptor associated kinase 1 antibody
    • Interleukin-1 receptor-associated kinase 1 antibody
    • IRAK antibody
    • IRAK-1 antibody
    • Irak1 antibody
    • IRAK1_HUMAN antibody
    • mPLK antibody
    • OTTHUMP00000026014 antibody
    • OTTHUMP00000026015 antibody
    • OTTHUMP00000026020 antibody
    • OTTHUMP00000180621 antibody
    • Pelle antibody
    • Pelle homolog antibody
    • Pelle-like protein kinase antibody
    • Plpk antibody
    see all

Anti-IRAK antibody 图像



  • Predicted band size : 77 kDa

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: IRAK1 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: Hek293 whole cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab55316 observed at 85 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab55316 was shown to specifically react with IRAK1 when IRAK1 knockout samples were used. Wild-type and IRAK1 knockout samples were subjected to SDS-PAGE. Ab55316 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

     

  • IRAK antibody (ab55316) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human testis.


  • Predicted band size : 77 kDa
    IRAK antibody (ab55316) at 1ug/lane + K-562 cell lysate at 25ug/lane.
  • ab55316 at 10 ug/ml staining IRAK in human Hella cells by Immunocytochemistry/ Immunofluorescence.

Anti-IRAK antibody (ab55316)参考文献

ab55316 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (8%)
Sample Human Cell lysate - whole cell (HEK293)
Specification HEK293
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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提交于 Aug 22 2013

Thank you for your enquiry regarding our products (ab55316, ab55318) and for sending the WB images. I am very sorry to hear that you are having problems with these two antibodies. As our Abpromise indicates, in the event that a product is not funct...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"