The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 - 4 µg/ml.
功能In addition to accelerate GTP gamma S binding by ARFs of all three classes, it appears to function preferentially as a guanine nucleotide exchange protein for ARF6, mediating internalisation of beta-1 integrin.
组织特异性Expressed in brain, ovary, heart, lung, liver, kidney and leukocytes. Moderate expression was also detected in lung, skeletal muscle, placenta, small intestine, pancreas, spleen and testis.
序列相似性Belongs to the BRAG family. Contains 1 IQ domain. Contains 1 PH domain. Contains 1 SEC7 domain.
细胞定位Cytoplasm. Nucleus. At steady state, may be preferentially cytosolic.
ab122442, at 4 µg/ml, staining IQSEC1 in Human A431 cell line, in green, by Immunoflouroscence. Fixation/Permeabilization: PFA/Triton X-100.
Anti-IQSEC1 antibody (ab122442)参考文献
This product has been referenced in:
Humphreys D et al. Arf6 coordinates actin assembly through the WAVE complex, a mechanism usurped by Salmonella to invade host cells. Proc Natl Acad Sci U S A110:16880-5 (2013).
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