概述

  • 产品名称Anti-IQGAP1抗体
    参阅全部 IQGAP1 一抗
  • 描述
    山羊多克隆抗体to IQGAP1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Dog, Chimpanzee, Rhesus monkey, Orangutan
  • 免疫原

    Synthetic peptide:

    C-KRQSGQTDPLQKEE

    , corresponding to internal sequence amino acids 357-370 of Human IQGAP1 NP_003861.1.

  • 阳性对照
    • Human kidney cell lysate, mouse kidney cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab99471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 189 kDa).

靶标

  • 功能Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth.
  • 组织特异性Expressed in the placenta, lung, and kidney. A lower level expression is seen in the heart, liver, skeletal muscle and pancreas.
  • 序列相似性Contains 1 CH (calponin-homology) domain.
    Contains 4 IQ domains.
    Contains 1 Ras-GAP domain.
    Contains 1 WW domain.
  • 结构域Regions C1 and C2 can either interact with nucleotide-free CDC42, or interact together, depending on the phosphorylation state of Ser-1443. When Ser-1443 is not phosphorylated, C1 and C2 interact, which prevents binding of nucleotide-free CDC42 and promotes binding of GTP-bound CDC42. Phosphorylation of Ser-1443 prevents interaction between C1 and C2, which opens the structure of the C-terminus and allows binding and sequestration of nucleotide-free CDC42 on both C1 and C2.
  • 翻译后修饰Phosphorylation of Ser-1443 by PKC prevents interaction between C1 and C2, allowing binding of nucleotide-free CDC42. Ser-1443 phosphorylation enhances the ability to promote neurite outgrowth.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HUMORFA01 antibody
    • IQ motif containing GTPase activating protein 1 antibody
    • IQGA1_HUMAN antibody
    • Iqgap1 antibody
    • KIAA0051 antibody
    • p195 antibody
    • Ras GTPase activating like protein 1 antibody
    • Ras GTPase-activating-like protein IQGAP1 antibody
    • RasGAP-like with IQ motifs antibody
    • SAR1 antibody
    see all

Anti-IQGAP1 antibody 图像

  • Anti-IQGAP1 antibody (ab99471) at 1 µg/ml + Human kidney cell lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 189 kDa

Anti-IQGAP1 antibody (ab99471)参考文献

ab99471 has not yet been referenced specifically in any publications.

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