The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明IP: Use at a concentration of 4 µg/ml (immunoprecipitated IQGAP1 from 500µg of a HepG2 RIPA lysate).
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 184 kDa. A goat anti-mouse secondary antibody conjugated to HRP can be used as secondary antibody together with a chemiluminescence detection system.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth.
组织特异性Expressed in the placenta, lung, and kidney. A lower level expression is seen in the heart, liver, skeletal muscle and pancreas.
结构域Regions C1 and C2 can either interact with nucleotide-free CDC42, or interact together, depending on the phosphorylation state of Ser-1443. When Ser-1443 is not phosphorylated, C1 and C2 interact, which prevents binding of nucleotide-free CDC42 and promotes binding of GTP-bound CDC42. Phosphorylation of Ser-1443 prevents interaction between C1 and C2, which opens the structure of the C-terminus and allows binding and sequestration of nucleotide-free CDC42 on both C1 and C2.
翻译后修饰Phosphorylation of Ser-1443 by PKC prevents interaction between C1 and C2, allowing binding of nucleotide-free CDC42. Ser-1443 phosphorylation enhances the ability to promote neurite outgrowth.