Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 1X PBS, pH 7.2
Concentration information loading...
Protein G purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight for isoform 2: 54 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Anti-IQ motif containing B1 antibody 图像
Western blot - IQ motif containing B1 antibody (ab69927)
All lanes :
Anti-IQ motif containing B1 antibody (ab69927) at 1/500 dilutionLane 1 :
IQ motif containing B1 transfected 293T cell lysateLane 2 :
non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilutionPredicted band size :
54 kDaObserved band size :
69 kDa (why is the actual band size different from the predicted?
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"