概述

  • 产品名称Anti-IPP抗体
    参阅全部 IPP 一抗
  • 描述
    兔多克隆抗体to IPP
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 432-481 (CCEMQGLIYV IGGISNEGIE LRSFEVYDPL SKRWSPLPPM GTRRAYLGVA) of Human IPP (NP_005888).

  • 阳性对照
    • Hela cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab81692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/1562500.

靶标

  • 相关性Intracisternal A particle promoted polypeptide (IPP) is a member of the kelch family of proteins, characterized by a 50 amino acid repeat which interacts with actin. The kelch domain of IPP consists of six C terminal tandem arranged repeats. IPP may play a role in organizing the actin cytoskeleton. IPP also contains an N terminal POZ protein protein interaction domain. The POZ domain (also called BTB domain) is present near the N terminus of a fraction of zinc finger proteins and in proteins that contain the pfam01344 motif such as kelch and pox virus proteins. The BTB/POZ domain mediates homomeric dimerization and in some instances heteromeric dimerization. POZ domains from several zinc finger proteins have been shown to mediate transcriptional repression and to interact with components of histone deacetylase co repressor complexes including N-coR and SMRT.
  • 细胞定位Cytoplasm, cytoskeleton.
  • 数据库链接
  • 别名
    • Actin binding protein IPP antibody
    • Intracisternal A particle promoted polypeptide antibody
    • IPP POZ antibody
    • Kelch like protein 27 antibody
    • KLHL27 antibody
    • MIPP protein antibody
    see all

Anti-IPP antibody 图像

  • Anti-IPP antibody (ab81692) at 1 µg/ml (in 5% skim milk / PBS buffer) + Hela cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 65 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 39 kDa,45 kDa. We are unsure as to the identity of these extra bands.

Anti-IPP antibody (ab81692)参考文献

ab81692 has not yet been referenced specifically in any publications.

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