概述

  • 产品名称Anti-IPMK抗体
    参阅全部 IPMK 一抗
  • 描述
    兔多克隆抗体to IPMK
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Recombinant protein fragment, corresponding to a region within amino acids 26 and 265 of Human IPMK (NP_689416).

  • 阳性对照
    • 293T, A431, H1299, HeLaS3, HepG2 and Raji cell lysates for WB and HeLa cells for IF.

性能

应用

Our Abpromise guarantee covers the use of ab96753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 47 kDa.
ICC/IF 1/100 - 1/200.

靶标

Anti-IPMK antibody 图像

  • Anti-IPMK antibody (ab96753) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size : 47 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96753 at 1/200 dilution. Lower image merged with DNA probe.

Anti-IPMK antibody (ab96753)参考文献

ab96753 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (FAO rat hepatoma cells)
Gel Running Conditions Reduced Denaturing (8%)
Loading amount 25 µg
Treatment 100 nM insulin for 5 minutes
Specification FAO rat hepatoma cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Sep 29 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"