Anti-IPL-1/STK13/Aurora C抗体(ab38299)


  • 产品名称Anti-IPL-1/STK13/Aurora C抗体
    参阅全部 IPL-1/STK13/Aurora C 一抗
  • 描述
    兔多克隆抗体to IPL-1/STK13/Aurora C
  • 经测试应用适用于: ELISA, WB, ICC/IF, IHC-Pmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to N terminal (M1) amino acids within 1-50 of Human IPL-1/STK13/Aurora C

  • 阳性对照
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal testis.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明ab38299 is purified through a protein G column and eluted out with both high and low pH buffers and neutralised immediately after elution then followed by dialysis against PBS
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab38299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
ICC/IF 1/100 - 1/500.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能May play a part in organizing microtubules in relation to the function of the centrosome/spindle pole during mitosis. May also contribute to the regulation of chromosome segregation and cytokinesis.
    • 组织特异性Isoform 1 and isoform 2 are expressed in testis. Elevated expression levels were seen only in a subset of cancer cells such as HepG2, HuH7 and HeLa cells. Expression is maximum at M phase.
    • 疾病相关Defects in AURKC are the cause of male infertility with large-headed multiflagellar polyploid spermatozoa (MIMPS) [MIM:243060]; also known as infertility associated with multi-tailed spermatozoa and excessive DNA.
    • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
      Contains 1 protein kinase domain.
    • 细胞定位Chromosome. Cytoplasm > cytoskeleton > spindle. Distributes in the condensed chromosomes during prophase to metaphase. After entering anaphase, there is a dissociation from separated chromosomes and a redistribution to midzone microtubules, and finally remaines in the midbody during cytokinesis.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AURKC antibody
      • AIE 2 antibody
      • Aie1 antibody
      • AIE2 antibody
      • AIK 3 antibody
      • AIK3 antibody
      • ARK 3 antibody
      • ARK-3 antibody
      • ARK3 antibody
      • Aur C antibody
      • AurC antibody
      • Aurkc antibody
      • AURKC_HUMAN antibody
      • aurora 3 antibody
      • Aurora C antibody
      • Aurora kinase C antibody
      • aurora related kinase 3 antibody
      • Aurora-related kinase 3 antibody
      • Aurora/Ipl1 related kinase 3 antibody
      • Aurora/Ipl1-related kinase 3 antibody
      • Aurora/Ipl1/Eg2 protein 2 antibody
      • EC antibody
      • Serine threonine protein kinase 13 antibody
      • serine/threonine kinase 13 (aurora/IPL1 like) antibody
      • serine/threonine protein kinase aurora C antibody
      • Serine/threonine-protein kinase 13 antibody
      • Serine/threonine-protein kinase aurora-C antibody
      • SPGF5 antibody
      • STK13 antibody
      see all

    Anti-IPL-1/STK13/Aurora C antibody 图像

    • IHC image of IPL-1/STK13/Aurora C staining in Human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab38299, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.


      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Immunofluorescence assay using HeLa cells expressing GFP-Aurora C was performed at different cellular mitotic stages, using ab38299 as primary antibody (A), GFP fluorescence (B), DAPI nuclear staining (C), and ab38299 merged to DAPI staining (column D).
    • All lanes : Anti-IPL-1/STK13/Aurora C antibody (ab38299) at 1/500 dilution

      Lane 1 : 293 cell lysate expressing Flag tag
      Lane 2 : 293 cell lysate expressing Flag tag Aurora A
      Lane 3 : 293 cell lysate expressing Flag tag Aurora B
      Lane 4 : 293 cell lysate expressing Flag tag Aurora C

      Predicted band size : 36 kDa
      Observed band size : 36 kDa
      Additional bands at : 20 kDa,75 kDa. We are unsure as to the identity of these extra bands.

      This image is courtesy of Drs. K. Sasai and S. Sen from the University of Texas MD Anderson Cancer Center (Houston, TX).

    Anti-IPL-1/STK13/Aurora C antibody (ab38299)参考文献

    This product has been referenced in:
    • Avo Santos M  et al. A role for Aurora C in the chromosomal passenger complex during human preimplantation embryo development. Hum Reprod 26:1868-81 (2011). WB, ICC/IF ; Human . Read more (PubMed: 21493633) »

    See 1 Publication for this product

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    The localization on our datasheets is always based automatically on Protein Knowledgebase ( Thank you for the specification!