使用敲除细胞株进行验证RabMAb

Anti-IP3 receptor抗体[EPR4537] (ab108517)

概述

  • 产品名称Anti-IP3 receptor抗体[EPR4537]
    参阅全部 IP3 receptor 一抗
  • 描述
    兔单克隆抗体[EPR4537] to IP3 receptor
  • 经测试应用适用于: WBmore details
    不适用于: Flow Cyt,ICC,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human ITPR1.

  • 阳性对照
    • Rat brain, SH-SY5Y, Mouse brain, and HeLa lysates
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab108517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 314 kDa.
  • 应用说明Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • 靶标

    • 功能Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate.
    • 组织特异性Widely expressed.
    • 疾病相关Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15) (SCA15) [MIM:606658]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA15 is an autosomal dominant cerebellar ataxia (ADCA). It is very slow progressing form with a wide range of onset, ranging from childhood to adult. Most patients remain ambulatory.
    • 序列相似性Belongs to the InsP3 receptor family.
      Contains 5 MIR domains.
    • 结构域The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region.
    • 翻译后修饰Phosphorylated by cAMP kinase. Phosphorylation prevents the ligand-induced opening of the calcium channels.
      Phosphorylated on tyrosine residues.
    • 细胞定位Endoplasmic reticulum membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 4 antibody
      • 5-trisphosphate receptor antibody
      • 5-trisphosphate receptor type 1 antibody
      • DKFZp313E1334 antibody
      • DKFZp313N1434 antibody
      • inositol 1 4 5 triphosphate receptor type 1 antibody
      • Inositol 1 4 5 trisphosphate Receptor Type 1 antibody
      • Inositol 1 antibody
      • InsP3R1 antibody
      • IP3 antibody
      • IP3 receptor antibody
      • IP3 receptor isoform 1 antibody
      • IP3R 1 antibody
      • IP3R antibody
      • IP3R1 antibody
      • ITPR 1 antibody
      • Itpr1 antibody
      • ITPR1_HUMAN antibody
      • SCA15 antibody
      • SCA16 antibody
      • SCA29 antibody
      • Type 1 inositol 1 4 5 trisphosphate receptor antibody
      • Type 1 inositol 1 antibody
      • Type 1 InsP3 receptor antibody
      see all

    Anti-IP3 receptor antibody [EPR4537] 图像



    • Predicted band size : 314 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: IP3 receptor knockout HAP1 cell lysate (20 µg)
      Lane 3: Human brain tissue lysate (20 µg)
      Lane 4: SH-SY5Y cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab108517 observed at 270 kDa. Red - loading control, ab18058, observed at 124 kDa.
      ab108517 was shown to recognize IP3 receptor when IP3 receptor knockout samples were used, along with additional cross-reactive bands. Wild-type and IP3 receptor knockout samples were subjected to SDS-PAGE. ab108517 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-IP3 receptor antibody [EPR4537] (ab108517) at 1/1000 dilution

      Lane 1 : Rat brain lysate
      Lane 2 : SH-SY5Y lysate
      Lane 3 : Mouse brain lysate
      Lane 4 : HeLa brain lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 314 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-IP3 receptor antibody [EPR4537] (ab108517)参考文献

    ab108517 has not yet been referenced specifically in any publications.

    Product Wall

    Please find here below the promised information about the ab108517 as well. I know it might not be useful to you anymore, but I received now the answer from the laboratory. As already mentioned, we do not have tested this antibody against bovin...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"