概述

  • 产品名称Anti-IP10抗体
    参阅全部 IP10 一抗
  • 描述
    兔多克隆抗体to IP10
  • 特异性This antibody does not cross-react with murine CXCL9 and CXCL11.
  • 经测试应用适用于: WB, ELISA, Neutralising, ICC/IFmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Highly pure (>98%) recombinant mIP-10 ( mouse Interferon Inducible Protein 10)

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab9938 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. To detect mIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIP-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use at an assay dependent concentration. To detect mIP-10 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIP-10.Western Blot:To detect mIP-10
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of mIP-10 (15.00 ng/ml), a concentration of 0.5-0.9 µg/ml of this antibody is required.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3.
  • 序列相似性Belongs to the intercrine alpha (chemokine CxC) family.
  • 翻译后修饰CXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Interferon gamma induced factor MOB1, mouse, homolog of antibody
    • Interferon gamma induced protein 10 antibody
    • 10 kDa interferon gamma induced protein antibody
    • 10 kDa interferon gamma-induced protein antibody
    • C X C motif chemokine 10 antibody
    • C7 antibody
    • Chemokine (C X C motif) ligand 10 antibody
    • Chemokine CXC motif ligand 10 antibody
    • Crg 2 antibody
    • CRG2 antibody
    • CXCL10 antibody
    • CXCL10(1-73) antibody
    • CXL10_HUMAN antibody
    • Gamma IP10 antibody
    • Gamma-IP10 antibody
    • gIP 10 antibody
    • GIP10 antibody
    • IFI10 antibody
    • INP 10 antibody
    • INP10 antibody
    • Interferon activated gene 10 antibody
    • Interferon activated gene 10 antibody
    • Interferon gamma induced cell line antibody
    • Interferon inducible cytokine IP 10 antibody
    • Interferon inducible cytokine IP10 antibody
    • IP 10 antibody
    • IP-10 antibody
    • Mob 1 antibody
    • MOB1 antibody
    • Protein 10 from interferon (gamma) induced cell line antibody
    • SCYB10 antibody
    • Small inducible cytokine B10 antibody
    • Small inducible cytokine B10 precursor antibody
    • Small inducible cytokine subfamily B (Cys X Cys) member 10 antibody
    • Small inducible cytokine subfamily B CXC member 10 antibody
    • Small inducible cytokine subfamily B, member 10 antibody
    • Small-inducible cytokine B10 antibody
    see all

Anti-IP10 antibody 图像

  • Western blot performed with ab9938 at 0.2 ug/ml
    Lane 1: MultiMark MultiColor Standard (Invitrogen)
    Lane 2: 250 ng Murine IP-10
    Lane 3: 125 ng Murine IP-10
    Lane 4: 62.5 ng Murine IP-10
    Lane 5: 31.25 ng Murine IP-10
    Lane 6: 15.625 ng Murine IP-10
    Lane 7: 7.8 ng Murine IP-10
    Lane 8: 3.9 ng Murine IP-10
    Lane 9: 1.95 ng Murine IP-10
    Lane 10: 0.975 ng Murine IP-10
    Lane 11: 0.4875 ng Murine IP-10
    Lane 12 0.24 ng Murine IP-10
  • Staining of colchicine injected mouse brain (including the hippocampus region) tissue. The primary antibody was incubated at 1.0 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein reagent. Optimal concentrations and conditions may vary.
  • Staining in colchicine injected mouse brain (including the hippocampus region) tissue. The primary antibody was incubated at 1.0 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein reagent. Optimal concentrations and conditions may vary.

Anti-IP10 antibody (ab9938)参考文献

This product has been referenced in:
  • Chan CC  et al. The role of interferon-? inducible protein-10 in a mouse model of acute liver injury post induced pluripotent stem cells transplantation. PLoS One 7:e50577 (2012). Neutralising ; Mouse . Read more (PubMed: 23227188) »
  • Kaur S  et al. Regulatory effects of mammalian target of rapamycin-activated pathways in type I and II interferon signaling. J Biol Chem 282:1757-68 (2007). Read more (PubMed: 17114181) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver, spleen)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH6 Sodium Citrate
Permeabilization No
Specification Liver, spleen
Blocking step Serum and BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 17 2016

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (liver, hepatocytes)
Specification liver, hepatocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jan 19 2015

The molecular weight for this protein is 8.7kDa on a western blot.

I hope this is helpful. Please let me know if you have any additional questions.

Thanks for running the experiment again with the diluted primary and secondary antibodies. A few points: 1. A couple other western blots of IP10 I have seen show detection of a band around 14kDa. I don't know what the molecular weight ma...

Read More

Your concerns are certainly valid ones and repeating the experiment with increased dilutions of the primary and secondary may just decrease all the bands equally. However, diluting the antibodies may instead serve to reduce the background and simul...

Read More

This antibody has been tested on a neutralisation assay (also known as a migration assay), so is suitable for the user.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"