概述

  • 产品名称Anti-IP10抗体
    参阅全部 IP10 一抗
  • 描述
    兔多克隆抗体to IP10
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 to the C-terminus of Human IP10.

    (Peptide available as ab47417.)

  • 阳性对照
    • This antibody gave a positive signal against Human recombinant IP10 full length protein

性能

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应用

Our Abpromise guarantee covers the use of ab47045 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 10 kDa (predicted molecular weight: 11 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3.
  • 序列相似性Belongs to the intercrine alpha (chemokine CxC) family.
  • 翻译后修饰CXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Interferon gamma induced factor MOB1, mouse, homolog of antibody
    • Interferon gamma induced protein 10 antibody
    • 10 kDa interferon gamma induced protein antibody
    • 10 kDa interferon gamma-induced protein antibody
    • C X C motif chemokine 10 antibody
    • C7 antibody
    • Chemokine (C X C motif) ligand 10 antibody
    • Chemokine CXC motif ligand 10 antibody
    • Crg 2 antibody
    • CRG2 antibody
    • CXCL10 antibody
    • CXCL10(1-73) antibody
    • CXL10_HUMAN antibody
    • Gamma IP10 antibody
    • Gamma-IP10 antibody
    • gIP 10 antibody
    • GIP10 antibody
    • IFI10 antibody
    • INP 10 antibody
    • INP10 antibody
    • Interferon activated gene 10 antibody
    • Interferon activated gene 10 antibody
    • Interferon gamma induced cell line antibody
    • Interferon inducible cytokine IP 10 antibody
    • Interferon inducible cytokine IP10 antibody
    • IP 10 antibody
    • IP-10 antibody
    • Mob 1 antibody
    • MOB1 antibody
    • Protein 10 from interferon (gamma) induced cell line antibody
    • SCYB10 antibody
    • Small inducible cytokine B10 antibody
    • Small inducible cytokine B10 precursor antibody
    • Small inducible cytokine subfamily B (Cys X Cys) member 10 antibody
    • Small inducible cytokine subfamily B CXC member 10 antibody
    • Small inducible cytokine subfamily B, member 10 antibody
    • Small-inducible cytokine B10 antibody
    see all

Anti-IP10 antibody 图像

  • Anti-IP10 antibody (ab47045) at 1 µg/ml + Recombinant human IP10 protein (ab9810) at 0.01 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 11 kDa
    Observed band size : 11 kDa
  • ab47045 staining human liver (healthy) sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed. Blocking of the sample was done with 5 minutes of peroxidase block and 10 minutes of protein block at 20°C, followed by staining with ab47045 at 1/50 for 45 minutes at 20°C. A HRP conjugated goat anti-rabbit polyclonal antibody was used as the secondary antibody.

    See Abreview

Anti-IP10 antibody (ab47045)参考文献

This product has been referenced in:
  • Ploquin MJ  et al. Elevated Basal Pre-infection CXCL10 in Plasma and in the Small Intestine after Infection Are Associated with More Rapid HIV/SIV Disease Onset. PLoS Pathog 12:e1005774 (2016). IHC-Fr ; Cynomolgus Monkey . Read more (PubMed: 27509048) »
  • Chmielewski S  et al. STAT1-dependent signal integration between IFN? and TLR4 in vascular cells reflect pro-atherogenic responses in human atherosclerosis. PLoS One 9:e113318 (2014). IHC-P ; Human . Read more (PubMed: 25478796) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Normal Liver)
Specification Normal Liver
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
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Verified customer

提交于 Sep 07 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"