Anti-Ionotropic Glutamate receptor 4 (phospho S862)抗体(ab61054)
概述
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产品名称Anti-Ionotropic Glutamate receptor 4 (phospho S862)抗体
参阅全部 Ionotropic Glutamate receptor 4 一抗 -
描述兔多克隆抗体to Ionotropic Glutamate receptor 4 (phospho S862)
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宿主Rabbit
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特异性Detects target only when phosphorylated at serine 862.
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经测试应用适用于: WB, ELISAmore details
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种属反应性与反应: Human
预测可用于: Mouse
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免疫原
Synthetic phosphopeptide derived from human ionotropic Glutamate Receptor 4 around the phosphorylation site of serine 862 (R-L-SP-I-T)
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+and Ca2+), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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纯化说明The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab61054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa). | |
| ELISA | 1/5000. |
靶标
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功能Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
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序列相似性Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA4 subfamily.
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翻译后修饰Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-611 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-837 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
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细胞定位Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
- Information by UniProt
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数据库链接
- Entrez Gene: 2893 Human
- Entrez Gene: 14802 Mouse
- Omim: 138246 Human
- SwissProt: P48058 Human
- SwissProt: Q9Z2W8 Mouse
- Unigene: 503743 Human
- Unigene: 209263 Mouse
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别名
- AMPA 4 antibody
- AMPA selective glutamate receptor 4 antibody
- AMPA-selective glutamate receptor 4 antibody
see all
图片
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Western blot - Anti-Ionotropic Glutamate receptor 4 (phospho S862) antibody (ab61054)All lanes : Anti-Ionotropic Glutamate receptor 4 (phospho S862) antibody (ab61054) at 1/500 dilution
Lane 1 : extracts from HepG2 cells treated with Forskolin (40nM, 30mins)
Lane 2 : extracts from HepG2 cells treated with Forskolin (40nM, 30mins) with immunizing phosphopeptide
Predicted band size: 102 kDa
Observed band size: 102 kDa
文献
ab61054 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"