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Fusion protein, corresponding to cytoplasmic C-terminal amino acids 834-883 of Rat Ionotropic Glutamate receptor 2 (P42262).
Our Abpromise guarantee covers the use of ab106515 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 99 kDa.|
|IHC-P||Use a concentration of 0.1 - 1 µg/ml.|
|IHC-Fr||Use a concentration of 0.1 - 1 µg/ml.|
|ICC||Use a concentration of 0.1 - 1 µg/ml.|
|ICC/IF||Use a concentration of 1 - 10 µg/ml.|
|Flow Cyt||Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
ab106515 Immunoprecipitating Ionotropic Glutamate receptor 2 in mouse hippocampus tissue lysate. 5µg of tissue lysate was incubated with primary antibody (1/100) and matrix (Protein G) for 24 hours at 4°C. For western blotting ab106515 (1/5000) was used to confirm successful immunoprecipation.
ab106515 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"