The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/100. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
Use at an assay dependent concentration.
功能Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.
序列相似性Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA2 subfamily.
翻译后修饰Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
ab52176 at 1/50 dilution staining Ionotropic Glutamate receptor 2 in human brain by Immunohistochemistry, paraffin embedded tissue, in the presence (right image) and absence (left image) of the immunizing peptide.
Western blot - Anti-Ionotropic Glutamate receptor 2 antibody (ab52176)
Bonnet CS et al. AMPA/kainate glutamate receptors contribute to inflammation, degeneration and pain related behaviour in inflammatory stages of arthritis. Ann Rheum DisN/A:N/A (2013).
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