Anti-Involucrin抗体[SY8] (ab20202)

概述

  • 产品名称Anti-Involucrin抗体[SY8]
    参阅全部 Involucrin 一抗
  • 描述
    小鼠单克隆抗体[SY8] to Involucrin
  • 特异性Keratinocytes of epidermis and other stratified squamous epithelia
  • 经测试应用适用于: IP, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human, Pig, Gorilla
  • 免疫原

    Pure involucrin from human keratinocytes.

性能

应用

Our Abpromise guarantee covers the use of ab20202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 68 kDa.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
  • 组织特异性Keratinocytes of epidermis and other stratified squamous epithelia.
  • 序列相似性Belongs to the involucrin family.
  • 翻译后修饰Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.
  • 细胞定位Cytoplasm. Constituent of the scaffolding of the cornified envelope.
  • Information by UniProt
  • 数据库链接
  • 别名
    • INVO_HUMAN antibody
    • Involucrin antibody
    • IVL antibody

Anti-Involucrin antibody [SY8] 图像

  • Ab20202 staining Human skin Staining is localised to the cytoplasm.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-Involucrin antibody [SY8] (ab20202)参考文献

ab20202 has not yet been referenced specifically in any publications.

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