Anti-Involucrin抗体[SY5] (ab68)

概述

  • 产品名称Anti-Involucrin抗体[SY5]
    参阅全部 Involucrin 一抗
  • 描述
    小鼠单克隆抗体[SY5] to Involucrin
  • 经测试应用适用于: ICC/IF, WB, IP, IHC-P, IHC-Fr, Flow Cytmore details
  • 种属反应性
    与反应: Dog, Human, Pig, Gorilla, Owl monkey
    不与反应: Mouse
  • 免疫原

    Pure involucrin from human keratinocytes.

  • 表位The epitope maps between codon 421-568 of human involucrin.
  • 阳性对照
    • MCF-7 cells. Localized to upper spinous and granular layers in Normal skin.
  • 常规说明

    Involucrin is the differentiation marker of human keratinocytes.
    ab81216 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

性能

应用

Our Abpromise guarantee covers the use of ab68 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 0.1 - 0.2 µg/ml.
IHC-Fr 1/100.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
  • 组织特异性Keratinocytes of epidermis and other stratified squamous epithelia.
  • 序列相似性Belongs to the involucrin family.
  • 翻译后修饰Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.
  • 细胞定位Cytoplasm. Constituent of the scaffolding of the cornified envelope.
  • Information by UniProt
  • 数据库链接
  • 别名
    • INVO_HUMAN antibody
    • Involucrin antibody
    • IVL antibody

Anti-Involucrin antibody [SY5] 图像

  • Immunohistochemical analysis of Formalin fixed paraffin embedded human tonsil section labelling Involucrin with ab68 at dilution of 0.1 ug/mL. 

  • ab68 at a 1/1000 dilution staining Involucrin in human normal skin tissue by Immunohistochemistry (formalin fixed, paraffin embedded sections), incubated for 2 hours at 21°C. Heat mediated antigen retrieval step performed using citrate buffer pH 6.0. Blocked with 10% serum for 30 minutes at 21°C. Secondary used at 1/100 polyclonal Donkey anti-mouse IgG conjugated to Alexa Fluor 594 (red).

    See Abreview

  • Immunohistochemical analysis of frozen Human artifical skin tissue labeling Involucrin with ab68 at 1/200 dilution.

  • ICC/IF image of ab68 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing A431 cells stained with ab68 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab68, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H&L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Involucrin antibody [SY5] (ab68)参考文献

This product has been referenced in:
  • Stinson SE  et al. D prostanoid receptor 2 (chemoattractant receptor-homologous molecule expressed on TH2 cells) protein expression in asthmatic patients and its effects on bronchial epithelial cells. J Allergy Clin Immunol 135:395-406 (2015). Read more (PubMed: 25312757) »
  • Shanmugam VK  et al. Utility of a human-mouse xenograft model and in vivo near-infrared fluorescent imaging for studying wound healing. Int Wound J 12:699-705 (2015). IHC ; Human . Read more (PubMed: 24373153) »

See all 13 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Skin)
Specification Skin
Blocking step Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative Acetone
Username

Abcam user community

Verified customer

提交于 Jul 14 2016

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (artificial skin)
Permeabilization Yes - Triton X-100
Specification artificial skin
Blocking step Blocking One as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 20 2015

Thank you for your patience.
I apologize for the delay, but I only heard back from the supplying lab today with the following information:

"Wehave unfortunately no data to explain the differeing bands seen against human tissue.
Read More

I am very sorry for the delayed response.

Despite numerous reminders the source of the antibody only replied to me today with the following information:

"We have no other information other than the codon sequence on the epitope f...

Read More

Thank you for contacting us.

I apologize for the wait, but I have not yet heard back from the lab. I have sent another email to follow up with them.

Thank you for your patience and understanding.

Thank you for contacting us.

I have contacted the lab for the questions you had, and will let you know their answer.

The database links for human and mouse proteins are as follows:

human: http://www.uniprot.org/uniprot/P07...

Read More
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Skin)
Specification Skin
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Mar 19 2012

Thank you for contacting us.

I have attached the MSDS for ab68.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Skin)
Specification Skin
Fixative Acetone
Permeabilization No
Blocking step Casein as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: 20°C
Username

Dr. Vaughan Feisst

Verified customer

提交于 Oct 23 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Normal Skin)
Specification Normal Skin
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrat, pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Feb 19 2010

1-10 of 14 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"