概述

  • 产品名称Anti-Involucrin抗体
    参阅全部 Involucrin 一抗
  • 描述
    兔多克隆抗体to Involucrin
  • 特异性ab53112 detects endogenous levels of total Involucrin protein.
  • 经测试应用适用于: ICC/IF, WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A synthetic peptide derived from human Involucrin.

  • 阳性对照
    • Human breast carcinoma tissue and extracts from HuvEc cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab53112 was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab53112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 21067603
WB 1/300 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.

靶标

  • 功能Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
  • 组织特异性Keratinocytes of epidermis and other stratified squamous epithelia.
  • 序列相似性Belongs to the involucrin family.
  • 翻译后修饰Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.
  • 细胞定位Cytoplasm. Constituent of the scaffolding of the cornified envelope.
  • Information by UniProt
  • 数据库链接
  • 别名
    • INVO_HUMAN antibody
    • Involucrin antibody
    • IVL antibody

Anti-Involucrin antibody 图像

  • All lanes : Anti-Involucrin antibody (ab53112) at 1/300 dilution

    Lane 1 : HuvEc cell extract
    Lane 2 : HuvEc cell extract with immunising peptide


    Predicted band size : 68 kDa
    Observed band size : 68 kDa
  • ab53112 staining human skin sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab53112 at 1/150 in TBS/BSA/azide for 16h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.

    See Abreview

  • Immunofluorescence analysis of Human skin xenografts carrying either control GFP (left) or SHH retrovirus (right), staining Involucrin (red) with ab53112.
  • ab53112 at 1/50 dilution staining Involucrin in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.

Anti-Involucrin antibody (ab53112)参考文献

This product has been referenced in:
  • Varone A  et al. Endogenous two-photon fluorescence imaging elucidates metabolic changes related to enhanced glycolysis and glutamine consumption in precancerous epithelial tissues. Cancer Res 74:3067-75 (2014). Read more (PubMed: 24686167) »
  • Ichida JK  et al. Notch inhibition allows oncogene-independent generation of iPS cells. Nat Chem Biol 10:632-9 (2014). Read more (PubMed: 24952596) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Skin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: ph6
Permeabilization No
Specification Skin
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 15 2015

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Mouse Tissue sections (Skin)
Specification Skin
Permeabilization Yes - 0,3 % Triton X
Fixative Perfusion fixed with paraformaldehyde and picric acid
Username

Abcam user community

Verified customer

提交于 Aug 11 2014

Thank you for calling about the issue you are having with the involucrin antibody ab53112. The amino acid sequence of the recombinant fragment that you are using as a positive control, ab73744, overlaps the sequence of the immunogen at 11 of 14 amino a...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Skin)
Specification Skin
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Mar 19 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (skin)
Specification skin
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: H-3300 Vector lab
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 01 2012

Thank you for your reply with further information. The image suggests the higher band may be the correct band as it is only present in the first four samples as expected. You previously describe a higher band at 120 kDa and I assuming this is that b...

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