The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 68 kDa.
功能Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
组织特异性Keratinocytes of epidermis and other stratified squamous epithelia.
序列相似性Belongs to the involucrin family.
翻译后修饰Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.
细胞定位Cytoplasm. Constituent of the scaffolding of the cornified envelope.
Paraffin embedded formalin fixed human skin tissue section stained with anti Involucrin (ab27495).
Anti-Involucrin antibody (ab27495)参考文献
This product has been referenced in:
Melo SP et al. Somatic correction of junctional epidermolysis bullosa by a highly recombinogenic AAV variant. Mol Ther22:725-33 (2014).
Read more (PubMed: 24390279) »
Lamb R & Ambler CA Keratinocytes propagated in serum-free, feeder-free culture conditions fail to form stratified epidermis in a reconstituted skin model. PLoS One8:e52494 (2013).
Read more (PubMed: 23326335) »