Anti-Inversin抗体(ab65187)

概述

  • 产品名称Anti-Inversin抗体
  • 描述
    兔多克隆抗体to Inversin
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Chicken, Cow, Dog, Chimpanzee
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Inversin.

    (Peptide available as ab73973.)

  • 阳性对照
    • This antibody gave a positive signal in the following Lysates: Mouse Liver Tissue K562 Whole Cell Jurkat Whole Cell HeLa Whole Cell HeLa Nuclear MEF1 Whole Cell

性能

应用

Our Abpromise guarantee covers the use of ab65187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.

靶标

  • 相关性Required for normal renal development and establishment of left-right axis. Probably acts as a molecular switch between different Wnt signaling pathways. Inhibits the canonical Wnt pathway by targeting cytoplasmic disheveled (DVL1) for degradation by the ubiquitin-proteasome. This suggests that it is required in renal development to oppose the repression of terminal differentiation of tubular epithelial cells by Wnt signaling. Binds calmodulin via its IQ domains. Interacts with microtubules. (from SwissProt).
  • 细胞定位Cytoplasm, cytoskeleton, spindle, membrane; Peripheral membrane protein, nucleus. Note=Associates with several components of the cytoskeleton including ciliary, random and polarized microtubules. During mitosis, it is recruited to mitotic spindle. Frequently membrane-associated, membrane localization is dependent upon cell-cell contacts and is redistributed when cell adhesion is disrupted after incubation of the cell monolayer with low-calcium/EGTA medium.
  • 数据库链接
  • 别名
    • INV antibody
    • Inversion of embryo turning homolog antibody
    • inversion of embryonic turning antibody
    • INVS antibody
    • Nephrocystin 2 antibody
    • Nephrocystin-2 antibody
    • Nephrocystin2 antibody
    • nephronophthisis 2 (infantile) antibody
    • NPH2 antibody
    • NPHP2 antibody
    see all

Anti-Inversin antibody 图像

  • All lanes : Anti-Inversin antibody (ab65187) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 6 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 118 kDa
    Observed band size : 118 kDa
    Additional bands at : 140 kDa,42 kDa,58 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes
  • ICC/IF image of ab65187 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65187, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 5µg/ml.
  • IHC image of Inversin staining in Human Cervical Cancer FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab65187, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

Anti-Inversin antibody (ab65187)参考文献

ab65187 has not yet been referenced specifically in any publications.

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