Horse, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster
Synthetic peptide corresponding to a region between residues 1000 and 1050 of human INTS5 (NP_085131.1)
HeLa and NIH3T3 whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
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Immunogen affinity purified
ab74405 was affinity purified using an epitope specific to INTS5 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 108 kDa.
Use at 2-5 µg/mg of lysate.
INTS5 is a subunit of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.
Nucleus. Membrane; Multi pass membrane protein.
integrator complex subunit 5 antibody
Western blot - Anti-INTS5 antibody (ab74405)
All lanes : Anti-INTS5 antibody (ab74405) at 0.4 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : NIH3T3 whole cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 108 kDa Observed band size: 108 kDa Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands. Exposure time: 10 seconds
Immunoprecipitation - Anti-INTS5 antibody (ab74405)
Detection of INTS5 by Western Blot of Immunprecipitate.
ab74405, at 1µg/ml, staining INTS5 in HeLa whole cell lysate immunoprecipitated using ab74405 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
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