1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.
Belongs to the Integrator subunit 4 family. Contains 8 HEAT repeats.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling INTS4 with ab75253 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - INTS4 antibody (ab75253)
All lanes : Anti-INTS4 antibody (ab75253) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 108 kDa Observed band size : 108 kDa
Exposure time : 30 seconds
Immunoprecipitation - INTS4 antibody (ab75253)
Detection of INTS4 by Western Blot of Immunprecipitate.
ab75253, at 1µg/ml, staining INTS4 in HeLa whole cell lysate immunoprecipitated using ab75253 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.