概述

  • 产品名称
  • 描述
    兔多克隆抗体to INTS3
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 975 and 1025 of human FLJ21991. Swiss-Prot Q68E01.

  • 阳性对照
    • Nuclear extract from HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab70451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明
    IP: Use at 1-4µg/ml lysate.
    WB: 1/1000 - 1/5000. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性
      Function: Component of the Integrator complex. The Integrator complex is involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. The SOSS complex associates with single-stranded DNA at DNA lesions and influences diverse endpoints in the cellular DNA damage response including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. The SOSS complex is required for efficient homologous recombination-dependent repair of double-strand breaks (DSBs) and ATM-dependent signaling pathways. In the SOSS complex, it is required for the assembly of the complex and for stabilization of the complex at DNA damage sites. Similarity: Belongs to the Integrator subunit 3 family.
    • 细胞定位
      Nuclear
    • 数据库链接
    • 别名
      • C1orf193 antibody
      • C1orf60 antibody
      • FLJ21919 antibody
      • INT 3 antibody
      • Int3 antibody
      • INT3_HUMAN antibody
      • Integrator complex subunit 3 antibody
      • Ints3 antibody
      • Sensor of single-strand DNA complex subunit A antibody
      • Sensor of ssDNA subunit A antibody
      • SOSS complex subunit A antibody
      • SOSS-A antibody
      see all

    Anti-INTS3 antibody 图像

    • Anti-INTS3 antibody (ab70451) at 0.33 µg/ml + HeLa nuclear extract at 25 µg

      Predicted band size : 118 kDa
      Observed band size : 118 kDa
    • 5mg HeLa cell nuclear extract treated with ab70451 at 4µg/5 mg lysate. Detection: Coomassie staining. The identity of the FLJ21919 band was verified by mass spectrometry.

    Anti-INTS3 antibody (ab70451)参考文献

    This product has been referenced in:
    • Stadelmayer B  et al. Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes. Nat Commun 5:5531 (2014). CHIPseq . Read more (PubMed: 25410209) »

    See 1 Publication for this product

    Product Wall

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Vascular smooth muscle cell)
    Permeabilization
    Yes - NP40
    Specification
    Vascular smooth muscle cell
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Jan 27 2017

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa cells and MCF10A cells)
    Loading amount
    15 µg
    Specification
    HeLa cells and MCF10A cells
    Gel Running Conditions
    Reduced Denaturing (12.5%)
    Blocking step
    Milk as blocking agent for 40 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Dr. Efrat Shema

    Verified customer

    提交于 Aug 02 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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