This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Anti-Intrinsic Factor抗体(ab91322)

概述

  • 产品名称Anti-Intrinsic Factor抗体
    参阅全部 Intrinsic Factor 一抗
  • 描述
    兔多克隆抗体to Intrinsic Factor

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Intrinsic Factor.

  • 阳性对照
    • This antibody gave a positive signal in Human Stomach Tumour tissue lysate.

性能

相关产品

靶标

  • 相关性Intrinsic factor promotes absorption of the essential vitamin Cobalamin (Cbl) in the ileum by specific receptor mediated endocytosis. Defects in the gene GIF are the cause of hereditary intrinsic factor deficiency (IFD) also called congenital pernicious anemia. IFD is an autosomal recessive disorder characterized by megaloblastic anemia.
  • 细胞定位Secreted
  • 数据库链接
  • 别名
    • Gastric Intrinsic Factor antibody
    • Gastric intrinsic factor (vitamin B synthesis) antibody
    • GIF antibody
    • IF antibody
    • IFMH antibody
    • INF antibody
    • Intrinsic factor antibody
    • TCM3 antibody
    • TCN3 antibody
    see all

Anti-Intrinsic Factor antibody 图像

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • Anti-Intrinsic Factor antibody (ab91322) at 1 µg/ml + Human Stomach Tumour at 10 µg

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 minutes
  • ICC/IF image of ab91322 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91322, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Intrinsic Factor antibody (ab91322)参考文献

ab91322 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"